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Qian Liu, Huiyang Zeng; The effect from β-chemokine to microglia and photoreceptor cells of mice in vitro. Invest. Ophthalmol. Vis. Sci. 2018;59(9):1491.
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To observe the activation of microglia and the photoreceptor cell apoptosis of mice induced by β-chemokines in vitro.
Activation of BV-2 cells were studied when β-chemokines (RANTES, MIP-1αand MIP-1β) were added. The cell death of 661w cells was observed when the supernatant of the activated BV-2 cells was added into the 661w cells. The 661w cell apoptosis was also studied when the β-chemokines were directly added. Met-RANTES (the inhibitor of β-chemokine) was added beforehand and above markers were studied.
The calcium influxes as well as release of ROS, NO by the BV-2 cells was increased (P<0.01) after adding β-chemokine. The 661w cell death was elevated by 30% after adding the supernatant of activated microglia. Above markers can be inhibited by the β-chemokine inhibitors (ROS P<0.01, No P=0.0187).There was no change when adding β-chemokine into 661w cells directly.
β-chemokines can activate microglia, which induce photoreceptor cell death by release of ROS or NO. Photoreceptor degeneration of RP mice may be protected by inhibition of β-chemokine inhibitors.
This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.
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