July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
The effect from β-chemokine to microglia and photoreceptor cells of mice in vitro
Author Affiliations & Notes
  • Qian Liu
    Beijing Ophthalmology & Visual Sciences Key Laboratory, Beijing Institute of Ophthalmology, Beijing, China
  • Huiyang Zeng
    Beijing Ophthalmology & Visual Sciences Key Laboratory, Beijing Institute of Ophthalmology, Beijing, China
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 1491. doi:
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      Qian Liu, Huiyang Zeng; The effect from β-chemokine to microglia and photoreceptor cells of mice in vitro. Invest. Ophthalmol. Vis. Sci. 2018;59(9):1491.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To observe the activation of microglia and the photoreceptor cell apoptosis of mice induced by β-chemokines in vitro.

Methods : Activation of BV-2 cells were studied when β-chemokines (RANTES, MIP-1αand MIP-1β) were added. The cell death of 661w cells was observed when the supernatant of the activated BV-2 cells was added into the 661w cells. The 661w cell apoptosis was also studied when the β-chemokines were directly added. Met-RANTES (the inhibitor of β-chemokine) was added beforehand and above markers were studied.

Results : The calcium influxes as well as release of ROS, NO by the BV-2 cells was increased (P<0.01) after adding β-chemokine. The 661w cell death was elevated by 30% after adding the supernatant of activated microglia. Above markers can be inhibited by the β-chemokine inhibitors (ROS P<0.01, No P=0.0187).There was no change when adding β-chemokine into 661w cells directly.

Conclusions : β-chemokines can activate microglia, which induce photoreceptor cell death by release of ROS or NO. Photoreceptor degeneration of RP mice may be protected by inhibition of β-chemokine inhibitors.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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