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Divya Sinha; Mitochondrial integrity and function in hPSC-derived RPE. Invest. Ophthalmol. Vis. Sci. 2018;59(9):1611. doi: https://doi.org/.
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Presentation Description :
Retinal pigment epithelium (RPE), a highly metabolically active monolayer of cells performing critical functions in the outer retina, is lost in several retinal degenerative disorders including age-related macular degeneration (AMD). Efforts are underway to develop transplantation strategies utilizing human pluripotent stem cell-derived RPE (hPSC-RPE) to replace these cells. RPE cells are known to be rich in mitochondria, however, the metabolic state of in vitro differentiated hPSC-RPE cells and their response to patient-specific in vivo stressors is not known. We investigated the mitochondrial integrity and function of these cells under baseline condition and conditions targeting mitochondrial respiration. Our results show that hPSC-RPE cells have healthy mitochondrial function, which can be affected by exposure to certain treatments. More specifically, the spare respiratory capacity (SRC), which is an indicator of a cell’s ability to manage cellular stress, can be modulated. This information has implications not only for cell survival and function post-transplantation but also in understanding and targeting mitochondrial function in early stages of disease.
This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.
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