July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Molecular imaging in the eye with photothermal optical coherence tomography
Author Affiliations & Notes
  • Melissa Skala
    University of Wisconsin - Madison, Madison, Wisconsin, United States
    Morgridge Institute for Research, Madison, Wisconsin, United States
  • Footnotes
    Commercial Relationships   Melissa Skala, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 1613. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Melissa Skala; Molecular imaging in the eye with photothermal optical coherence tomography. Invest. Ophthalmol. Vis. Sci. 2018;59(9):1613. doi: https://doi.org/.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Presentation Description : Optical coherence tomography (OCT) is a critical tool to evaluate eye diseases, and to guide eye surgery. OCT provides rich information on retinal structure, but lacks molecular contrast. We have developed photothermal OCT (PT-OCT) to provide molecular contrast in the eye, specifically to map endogenous absorbers such as melanin and exogenous absorbers such as indocyanine green. We have validated that the PT-OCT signal in the retinal pigment epithelium is due to melanin, using albino and wild-type mice, with additional validations in CRISPR-modified zebrafish. We have further confirmed that the PT-OCT signal is linear with absorber concentration, thus enabling quantitative in vivo imaging of melanin concentrations in the eye. Additionally, we have confirmed that PT-OCT is sensitive to indocyanine green, a common contrast agent in ophthalmic imaging, using ex vivo eyes. Overall, PT-OCT offers promise for studying molecular changes in animal models of eye disease, and could be a powerful tool for patient monitoring and surgical interventions.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.


This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.