July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Generation and purification of functional corneal endothelium-like cells differentiated from human embryonic stem cells
Author Affiliations & Notes
  • Xin Xia
    Ophthalmology, Stanford University, Palo Alto, California, United States
  • Kun-Che Chang
    Ophthalmology, Stanford University, Palo Alto, California, United States
  • Olga Kuzmenko
    Ophthalmology, Stanford University, Palo Alto, California, United States
  • Noelia J Kunzevitzky
    Ophthalmology, Stanford University, Palo Alto, California, United States
    Emmecell, Menlo Park , California, United States
  • Catalina Sun
    Ophthalmology, Stanford University, Palo Alto, California, United States
    Shiley Eye Center, San Diego, California, United States
  • Xiong Zhang
    Shiley Eye Center, San Diego, California, United States
  • Kevin Tenerelli
    Shiley Eye Center, San Diego, California, United States
  • Jeffrey L Goldberg
    Ophthalmology, Stanford University, Palo Alto, California, United States
  • Footnotes
    Commercial Relationships   Xin Xia, None; Kun-Che Chang, None; Olga Kuzmenko, None; Noelia Kunzevitzky, Emmecell (E), Emmecell (P); Catalina Sun, None; Xiong Zhang, None; Kevin Tenerelli, None; Jeffrey Goldberg, None
  • Footnotes
    Support  California Institute of Regenerative Medicine (DISC1-08848), the National Eye Institute (P30-EY026877 and P30-EY022125) and Research to Prevent Blindness, Inc.
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 2256. doi:
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    • Get Citation

      Xin Xia, Kun-Che Chang, Olga Kuzmenko, Noelia J Kunzevitzky, Catalina Sun, Xiong Zhang, Kevin Tenerelli, Jeffrey L Goldberg; Generation and purification of functional corneal endothelium-like cells differentiated from human embryonic stem cells. Invest. Ophthalmol. Vis. Sci. 2018;59(9):2256.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To restore endothelial function in patients with corneal endothelium dysfunction through cell transplant therapies, here we study differentiation of human embryonic stem cells (hESCs) into corneal endothelial cells (HCECs), and their properties of barrier and pump function.

Methods : A two-step method was used first to differentiate stem cells into neural crest cells (NCC), and then to induce maturation to HCEC-like cells. Cell morphology, phenotypic markers including expression of specific markers, and barrier functional assays assessed by transendothelial electrical resistance (TEER) were detected along a time course after differentiation.

Results : Differentiated hESC-derived HCECs demonstrated similar polygonal morphology as canonical primary HCECs. HCEC-specific markers, including zonula occludens-1 (ZO-1), sodium-potassium ATPase (Na+-K+-ATPase) and collagen VIII, showed similar expression patterns in hESC-derived HCEC-like cells. Cell sorting using different surface markers revealed that subpopulations with double-positive CD56 and CD166 marker expression showed higher barrier function by TEER than subpopulations positive for only a single HCEC marker.

Conclusions : Human stem cells can generate a large yield of HCEC-like progeny that carry phenotypic and functional measures similar to primary HCECs.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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