July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Using Genipin-crosslinked Acellular Porcine Corneal Stroma for Individual Design for Cosmetic Corneal Lens Implants
Author Affiliations & Notes
  • Zhao Liu
    Department of Ophthalmology, The First Affiliated Hospital of Xi’an Jiaotong University, Xi'an, Shaanxi, China
  • Cheng Pei
    Department of Ophthalmology, The First Affiliated Hospital of Xi’an Jiaotong University, Xi'an, Shaanxi, China
  • Footnotes
    Commercial Relationships   Zhao Liu, None; Cheng Pei, None
  • Footnotes
    Support  National Natural Science Foundation of China, Young Scientist Grant (81400380)
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 2271. doi:
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      Zhao Liu, Cheng Pei; Using Genipin-crosslinked Acellular Porcine Corneal Stroma for Individual Design for Cosmetic Corneal Lens Implants. Invest. Ophthalmol. Vis. Sci. 2018;59(9):2271.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Corneal leukoma is a stromal opacity caused by cornea diseases. It not only impairs vision but also damages the appearance of a person’s eyes and face. For them, cosmetic treatment is of great significance. Cosmetic contact lens and cosmetic keratopigmentation, as the main treatments for corneal imperfections at present, are limited in clinical applications due to a number of crucial flaws. The surgical techniques of keratophakia and epikeratophakia have been fully validated and affirmed. If refractive corneal lens implant (RCLI) is replaced by cosmetic corneal lens implant (CCLI), the implementation of which can fully meet the coverage requirements of corneal leukoma, the appearance of the affected eyes can thereby be improved. This study aims to get CCLIs with various coloration for the individual design by adjusting genipin crosslinking parameters under the instructions of regression equations.

Methods : Porcine eyes were made to nature porcine cornea (NPC). NPC was immersed in decellularization bicarbonate-mixed salt solution. After decellularization procedures, CCLIs was crosslinked in different genipin reaction conditions. The eXactTM Scan(X-Rite, Incorporated, US)was used to record chroma of CCLIs.

Results : 1. Chroma - reaction time regression analysis shows(time: 0.5 - 5 h):
L = 79.063 - 6.457 × time (P < 0.05, R2 = 0.798)
a = - 1.598 - 1.003 × time (P < 0.05, R2 = 0.846)
b = 11.779 - 2.348 × time (P < 0.05, R2 = 0.868)
2. Chroma - genipin concentration regression analysis shows(concentration: 0.1 - 1.0):
L = 78.239 - 28.466 × concentration (P < 0.05, R2 = 0.840)
a = - 1.204 - 8.108 × concentration + 4.742 × concentration3 (P < 0.05, R2 = 0.660)
b = 7.410 - 31.502 × concentration2 + 28.757 × concentration3 (P < 0.05, R2 = 0.623)
3. Chroma - pH regression analysis shows(pH: 0 - 13):
L = 86.512 - 5.243 × pH (P < 0.05, R2 = 0.867)
a = 2.116 - 3.359 × pH + 0.424 × pH2 - 0.012 × pH3 (P < 0.05, R2 = 0.617)
b = 2.497 - 1.898 Cos pH (P < 0.05, R2 = 0.267)

Conclusions : 1. Color of CCLI changes by adjusting genipin crosslinking parameters (time, concentration and pH).
2. Based on Chroma - reaction parameters regression equations, it is hopeful to get CCLIs with various coloration matching iris colors of different persons. It is a magical cure for leukoma to get the individual design for cosmetic treatment.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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