Abstract
Purpose :
Previous research has shown changes in blood composition depending on the food intake. There is no evidence whether these changes can also affect the quality of autologous eyedrops. Our study goal is to assess if IL-6 concentration in 3 different hemoderivate eye drops varies depending on the moment of blood extraction, prior to or after eating.
Methods :
Blood was extracted from 10 healthy volunteers under 2 specific circumstances: after a fasting period (at least 8 hours) and postprandially (3 hours and a half after food intake). All 10 participants had the same hipercaloric fatty meal. Blood was processed in 3 different ways in order to obtain 3 different autologous eye drops: autologous serum, plasma rich in growth factors and platelet rich plasma. IL-6 concentration was measured by colorimetric method Enzyme Linked Immuno Sorbent Assay (ELISA).
Results :
Postprandial situation of the patient while blood is extracted increases IL-6 concentracion in the final composition of all 3 studied hemoderivates, but not significantly (P>0.05). PRP elaboration technique induces slighter increase of IL-6 concentracion, but differences are not statistically significant (P>0.05) with the other 2 hemoderivates.
Conclusions :
Although these eye drops are usually used in ocular inflammatory diseases and a slight increase of IL-6 concentration is noticed when blood is extracted after food intake, the differences are not statistically significant. In conclusion, contrary to the generalized recommendation and in the absence of further studies, blood extraction could be performed in a postprandial situation. Further research would be needed to determine whether this situation has a clinical repercussion.
This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.