July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Comprehensive transcriptome analyses of ABCB5-positive limbal stem cells.
Author Affiliations & Notes
  • Yuzuru Sasamoto
    Department of Medicine, Brigham and Women's Hospital, Brookline, Massachusetts, United States
  • Gabriel Gonzalez
    Department of Medicine, Brigham and Women's Hospital, Brookline, Massachusetts, United States
    Medicine, VA Boston Healthcare System, Boston, Massachusetts, United States
  • Bruce Ksander
    Mass Eye & Ear, Schepens Eye Research Institute, Boston, Massachusetts, United States
  • Markus Hermann Frank
    Transplant Research Program, Boston Children's Hospital, Boston, Massachusetts, United States
  • Natasha Frank
    Department of Medicine, Brigham and Women's Hospital, Brookline, Massachusetts, United States
    Medicine, VA Boston Healthcare System, Boston, Massachusetts, United States
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 2288. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Yuzuru Sasamoto, Gabriel Gonzalez, Bruce Ksander, Markus Hermann Frank, Natasha Frank; Comprehensive transcriptome analyses of ABCB5-positive limbal stem cells.. Invest. Ophthalmol. Vis. Sci. 2018;59(9):2288.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : We have previously shown that ATP-binding cassette (ABC) superfamily member B5 (ABCB5) identifies adult limbal stem cells with the ability to restore and maintain the corneal epithelium upon transplantation. Here we hypothesized that adult ABCB5(+) stem cells display a unique molecular phenotype.

Methods : RNA-seq transcriptome analyses were performed using the SMART-seq protocol on Abcb5(+) and Abcb5(-) flow cytometry-sorted corneal epithelial cells freshly isolated from 4-week-old mice (n=4 groups, 5 mice per group).

Results : FACS analyses revealed that Abcb5(+) cells comprised 3.1±1.5% (mean±SD) of dissociated corneal epithelial cells. Principal component analysis (PCA) of RNA-seq-determined gene expression revealed separation of samples into two groups according to Abcb5 expression status. The mesenchymal stem cell-expressed genes CD73, CD166, and CXCR4 were enriched in Abcb5(+) cells, whereas the epithelial differentiation markers Connexin 43 and Desmoglein 3 (DSG3) were upregulated in Abcb5(-) cells.

Conclusions : Transcriptome analyses show that Abcb5(+) cells represent a molecularly distinct adult stem cell population with a unique gene profile reminiscent of mesenchymal stem cell phenotype.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×