July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Comprehensive transcriptome analyses of ABCB5-positive limbal stem cells.
Author Affiliations & Notes
  • Yuzuru Sasamoto
    Department of Medicine, Brigham and Women's Hospital, Brookline, Massachusetts, United States
  • Gabriel Gonzalez
    Department of Medicine, Brigham and Women's Hospital, Brookline, Massachusetts, United States
    Medicine, VA Boston Healthcare System, Boston, Massachusetts, United States
  • Bruce Ksander
    Mass Eye & Ear, Schepens Eye Research Institute, Boston, Massachusetts, United States
  • Markus Hermann Frank
    Transplant Research Program, Boston Children's Hospital, Boston, Massachusetts, United States
  • Natasha Frank
    Department of Medicine, Brigham and Women's Hospital, Brookline, Massachusetts, United States
    Medicine, VA Boston Healthcare System, Boston, Massachusetts, United States
  • Footnotes
    Commercial Relationships   Yuzuru Sasamoto, Alcon Japan Ltd. (F), Japan Eye Bank association (F), Kanae Foundation for the Promotion of Medical Science (F); Gabriel Gonzalez, None; Bruce Ksander, Rheacell GmbH & Co. KG (P), Ticeba GmbH (P); Markus Frank, Rheacell GmbH & Co. KG (P), Rheacell GmbH & Co. KG (C), Ticeba GmbH (P), Ticeba GmbH (C); Natasha Frank, Rheacell GmbH & Co. KG (P), Ticeba GmbH (P)
  • Footnotes
    Support  NIH/NEI 1RO1EY025794-01A1 grant to N.F., B.K and M.H.F, NIH/NEI3R01EY025794-01A1S1 Minority Supplement Award to G.G., NIH/NEI Schepens Core Grant P30EY003790 to B.K., VA RR&D 1I01RX000989 Merit Review Award and an HSCI seed grant award to N.F., the Kanae Foundation for the Promotion of Medical Science (Tokyo, Japan), Alcon Japan Ltd. (Tokyo, Japan) and Japan Eye Bank Association (Tokyo, Japan) to Y.S.
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 2288. doi:
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    • Get Citation

      Yuzuru Sasamoto, Gabriel Gonzalez, Bruce Ksander, Markus Hermann Frank, Natasha Frank; Comprehensive transcriptome analyses of ABCB5-positive limbal stem cells.. Invest. Ophthalmol. Vis. Sci. 2018;59(9):2288.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : We have previously shown that ATP-binding cassette (ABC) superfamily member B5 (ABCB5) identifies adult limbal stem cells with the ability to restore and maintain the corneal epithelium upon transplantation. Here we hypothesized that adult ABCB5(+) stem cells display a unique molecular phenotype.

Methods : RNA-seq transcriptome analyses were performed using the SMART-seq protocol on Abcb5(+) and Abcb5(-) flow cytometry-sorted corneal epithelial cells freshly isolated from 4-week-old mice (n=4 groups, 5 mice per group).

Results : FACS analyses revealed that Abcb5(+) cells comprised 3.1±1.5% (mean±SD) of dissociated corneal epithelial cells. Principal component analysis (PCA) of RNA-seq-determined gene expression revealed separation of samples into two groups according to Abcb5 expression status. The mesenchymal stem cell-expressed genes CD73, CD166, and CXCR4 were enriched in Abcb5(+) cells, whereas the epithelial differentiation markers Connexin 43 and Desmoglein 3 (DSG3) were upregulated in Abcb5(-) cells.

Conclusions : Transcriptome analyses show that Abcb5(+) cells represent a molecularly distinct adult stem cell population with a unique gene profile reminiscent of mesenchymal stem cell phenotype.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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