July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Geographic Atrophy: Correlation Between Confocal Scanning Laser Ophthalmoscopy (SLO), Histology and Immunohistology of Retinal Sections in the Region of Expanding Lesions.
Author Affiliations & Notes
  • Vera L Bonilha
    Ophthalmology, Cole Eye Inst/Cleveland Clin Lerner Ctr, Cleveland, Ohio, United States
  • Brent A Bell
    Ophthalmology, Cole Eye Inst/Cleveland Clin Lerner Ctr, Cleveland, Ohio, United States
  • Mary E Rayborn
    Ophthalmology, Cole Eye Inst/Cleveland Clin Lerner Ctr, Cleveland, Ohio, United States
  • Joe G Hollyfield
    Ophthalmology, Cole Eye Inst/Cleveland Clin Lerner Ctr, Cleveland, Ohio, United States
  • Stephanie A Hagstrom
    Ophthalmology, Cole Eye Inst/Cleveland Clin Lerner Ctr, Cleveland, Ohio, United States
  • Gayle J Pauer
    Ophthalmology, Cole Eye Inst/Cleveland Clin Lerner Ctr, Cleveland, Ohio, United States
  • Footnotes
    Commercial Relationships   Vera Bonilha, None; Brent Bell, None; Mary Rayborn, None; Joe Hollyfield, None; Stephanie Hagstrom, None; Gayle Pauer, None
  • Footnotes
    Support  Unrestricted Grant Award from Research to Prevent Blindness (RPB1508DM), Foundation Fighting Blindness Center Grant (CCMM08120584CCF), Wolf Family Foundation, BrightFocus Foundation (M2016079), NEI/NIH P30 Core Center Grant (IP30EY025585) and Foundation Fighting Blindness
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 2443. doi:
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      Vera L Bonilha, Brent A Bell, Mary E Rayborn, Joe G Hollyfield, Stephanie A Hagstrom, Gayle J Pauer; Geographic Atrophy: Correlation Between Confocal Scanning Laser Ophthalmoscopy (SLO), Histology and Immunohistology of Retinal Sections in the Region of Expanding Lesions.. Invest. Ophthalmol. Vis. Sci. 2018;59(9):2443.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Geographic atrophy (GA) represents the atrophic late-stage of dry AMD. AMD pathology affects the RPE physiology and results in several noticeable morphological features in these cells. Funduscopy images identify hypopigmented areas, in which larger choroidal vessels may become visible due to the absence of the RPE and the choriocapillaris. Visible light SLO autofluorescence (VAF-SLO) imaging visualizes the accumulation of the autofluorescent lipids composing lipofuscin at the level of the RPE monolayer in patients. Whereas near-infrared SLO autofluorescence (IRAF-SLO) has been introduced to visualize the distribution of melanin. Here, we characterized the GA from several donors by VAF- and IRAF-SLO and correlated it to histological and immunohistological findings of retinal sections.

Methods : SLO, and fundus macrography (FM) were used to identify and measure the extent of GA lesions from 28 fixed donor eyes. Adjacent areas of the retina/RPE/choroid from the margins of the GA border were cut to generate four smaller fragments: two were processed for epon embedding while the others were processed for cryosectioning and immunofluorescence in the green-red and near-IR (NIR) channels that closely matched SLO images. Cryosections were also reacted with antibodies to activated microglia (anti-Iba-1). These donor eyes were compared to several age-matched normal eyes in the perimacula. DNA was obtained from blood samples or fixed eye tissue of the donors and genotyped for single nucleotide polymorphisms (SNPs) previously shown to be associated with GA (rs2842992, near SOD2; rs1789110, near MBP; rs722782, near C80rf42).

Results : The majority of the GA donors displayed association with at least two of the risk alleles on SOD2, MBP, and C80rf42. The cytoplasm of RPE cells at the GA margins contained similar amounts of fluorescent granules visible in both NIR and green-red channels. RPE cells at the border of the GA display loss of apical localization of dark pigmented non-fluorescent granules. Borders of medium size GA lesions are enriched in Iba-1 positive cells.

Conclusions : GA eyes can be sub-grouped together based on the IRAF-SLO, presence of distribution of fluorescent granules within the RPE, and presence of microglia cells.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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