July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Neuroprotection of photoreceptors bearing the P23H mutation in Rhodopsin
Author Affiliations & Notes
  • Valeria Marigo
    Life Sciences, Univ of Modena and Reggio Emilia, Modena, Italy
  • Antonella Comitato
    Life Sciences, Univ of Modena and Reggio Emilia, Modena, Italy
  • Clara La Marca
    Life Sciences, Univ of Modena and Reggio Emilia, Modena, Italy
  • Davide Schiroli
    Life Sciences, Univ of Modena and Reggio Emilia, Modena, Italy
  • Preeti Subramanian
    Laboratory of Retinal Cell and Molecular Biology, NIH-NEI, Bethesda, Maryland, United States
  • Manel Llado
    Telethon Institute of Genetics and Medicine, Naples, Italy
  • S Patricia Becerra
    Laboratory of Retinal Cell and Molecular Biology, NIH-NEI, Bethesda, Maryland, United States
  • Alberto Auricchio
    Telethon Institute of Genetics and Medicine, Naples, Italy
  • Footnotes
    Commercial Relationships   Valeria Marigo, None; Antonella Comitato, None; Clara La Marca, None; Davide Schiroli, None; Preeti Subramanian, None; Manel Llado, None; S Patricia Becerra, None; Alberto Auricchio, None
  • Footnotes
    Support  Italian Telethon Foundation GGP14180, Fondazione Roma (call for proposal 2013 sulla Retinite Pigmentosa) and the Intramural Research Program of the NEI-NIH
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 2488. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Valeria Marigo, Antonella Comitato, Clara La Marca, Davide Schiroli, Preeti Subramanian, Manel Llado, S Patricia Becerra, Alberto Auricchio; Neuroprotection of photoreceptors bearing the P23H mutation in Rhodopsin. Invest. Ophthalmol. Vis. Sci. 2018;59(9):2488. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Autosomal dominant Retinitis Pigmentosa (adRP), an inherited degenerative disease causing blindness in later life, is frequently caused by dominant mutations in the rhodopsin gene (RHO). We previously characterized cell death pathways activated in a mouse model expressing the P23H RHO mutation and found to be linked to increased intracellular calcium, activation of calpains and Apoptosis inducing factor (Aif) as well as activation of ER-stress and caspase 7. Given that therapies for adRP are still not available, neuroprotection was evaluated as a novel therapy based on PEDF that was reported to be downregulated during retinal degeneration.

Methods : In vivo treatment of RhoP23H/+ mice were performed by intraocular injections of AAV expressing either Pigment Epithelium-derived Factor (PEDF) or a small peptide from the neuroprotection domain of PEDF (17mer[H105A]). Neuroprotection was assessed by histological analysis as well as molecular analysis by western blotting and immunofluorescence of cell death pathways.

Results : We developed different viral systems to restore PEDF levels in the degenerating retina. We tested neuroprotection by intravitreal or subretinal injections of AAV expressing PEDF or 17mer[H105A]. We found that PEDF protected more than 40% of photoreceptors from cell death in the RhoP23H/+ mutant retina and the 17mer[H105A] was even more efficient. Interestingly, PEDF was found to reduce activation of calpains and of the apoptosis inducing factor (Aif) involved in photoreceptor cell demise.

Conclusions : PEDF demonstrated to act at early stages of photoreceptor cell death. Long-term exposure to PEDF will be discussed.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×