July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Neurodegenerative changes in retina after mild traumatic brain injury: Protective role of paracrine secretions of adipose derived stem cells
Author Affiliations & Notes
  • Kumar Abhiram Jha
    Opthalmology, University of Tennessee Health science center, Memphis, Tennessee, United States
  • RAJI RAJESH LENIN
    Opthalmology, University of Tennessee Health science center, Memphis, Tennessee, United States
  • Sally L Elshaer
    Opthalmology, University of Tennessee Health science center, Memphis, Tennessee, United States
  • Jordy Gentry
    Opthalmology, University of Tennessee Health science center, Memphis, Tennessee, United States
  • Nobel Del Mar
    Anatomy & Neurobiology, University of Tennessee Health science center, Memphis, Tennessee, United States
  • TJ Hollingsworth
    Neuroscience Institute, UTHSC, Memphis, Tennessee, United States
  • Anton Reiner
    Anatomy & Neurobiology, University of Tennessee Health science center, Memphis, Tennessee, United States
  • Rajashekhar Gangaraju
    Opthalmology, University of Tennessee Health science center, Memphis, Tennessee, United States
  • Footnotes
    Commercial Relationships   Kumar Abhiram Jha, None; RAJI RAJESH LENIN, None; Sally Elshaer, None; Jordy Gentry, None; Nobel Del Mar, None; TJ Hollingsworth, None; Anton Reiner, None; Rajashekhar Gangaraju, Cell Care Therapeutics, INC. (F)
  • Footnotes
    Support  DOD Grant W81XWH-16-1-0778
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 2501. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Kumar Abhiram Jha, RAJI RAJESH LENIN, Sally L Elshaer, Jordy Gentry, Nobel Del Mar, TJ Hollingsworth, Anton Reiner, Rajashekhar Gangaraju; Neurodegenerative changes in retina after mild traumatic brain injury: Protective role of paracrine secretions of adipose derived stem cells. Invest. Ophthalmol. Vis. Sci. 2018;59(9):2501.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Mild traumatic brain (mTBI) injury is commonly experienced in battlefield, accidents and full contact sports, which affect vision. Inflammation-induced neurodegeneration is expected to play a major role in such visual deficits. In this study, we hypothesized that anti-inflammatory proteins released by adipose-derived stem cells (ADSC) can rescue retinal damage and improve visual function.

Methods : About 12 weeks old C57Bl/6 mice were subjected to 50-psi air pulse on the left side of the head, resulting in an mTBI. Sham-blast mice served as control. After blast injury, 1 µl of human ASDC conditioned medium (ADSC-CM) was delivered intravitreally. One month following injection, ocular function was assessed followed by immunohistological analysis. Efficacy of ADSC-CM in normalizing retinal vascular permeability was assessed by means of trans-endothelial resistance (TER) in the presence of TNF-α (1 ng/mL).

Results : Blast injury mice demonstrated decrease in visual acuity compared to the sham (0.302±0.01 v/s 0.39±0.01 c/d, p<0.001), with a significant improvement observed with ADSC-CM (0.376±0.007 c/d, p<0.001). Similarly, the contrast sensitivity of blast mice showed an increase in the contrast needed to detect 0.042 c/d (79.09±11.62 v/s 4.73±0.41, p<0.0001), with a significant improvement observed with ADSC-CM (79.09±11.62 v/s 41.23±8.42, p<0.001). Blast injury also resulted in a decrease in “b” wave amplitude compared to sham (368.2±40 v/s Blast, 409±33.5 mV, 25cd.s.m2 flash intensity, p<0.04), and improved by ADSC-CM, though it did not reach statistical significance (428.7±47.3 mV, p>0.05). Immunohistological analysis of retina demonstrated increased expression of GFAP in blast group as compared to sham with a reduction observed with ADSC-CM (p<0.04). Retinal endothelial cells exposed to TNF-α showed a reduction in barrier integrity as evidenced by decreased TER (TNF, 0.399±0.03; Control, 1.0±0.0 A.U., p<0.02), which was rescued with ADSC-CM (0.596±0.04 A.U., p<0.03).

Conclusions : Our studies suggest that blast injury leads to increased retinal degeneration, which could be ameliorated by ADSC-CM possibly through glial deactivation. Future studies are needed to explore the relationship of anti-inflammatory factors secreted in ADSC-CM with glial activation as a possible mechanism of the observed therapeutic benefit in blast-associated visual deficits.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×