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Yunli Ling, Shuo Yu, Cui Mao, Hong Lu; Deferentially Expressed Gene Analysis of HLA-B27-associated Acute Anterior Uveitis Families. Invest. Ophthalmol. Vis. Sci. 2018;59(9):2517.
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The aim of this study is to investigate deferentially expressed genes in human leukocyte antigen(HLA) B27-associated acute anterior uveitis families.
Study four families with HLA-B27 positive acute anterior uveitis (AAU) patients and their HLA-B27-positive blood relatives were included in the study. Their peripheral blood monocytes were isolated and stimulated by the Cholera vibrio lipopolysaccharide (LPS). Gene expression microarrays were used to identify the differentially expressed genes (DEGs), and the DEGs were testified by real-time PCR and analyzed by a series of bioinformatics-based techniques such as Gene Ontology (GO), Pathway analysis, Signal-Net analysis and Gene Relation Network (Gene-Rel-Net).
The gene expression microarrays analysis revealed 597 DEGs, including 258 up-regulated genes and 339 down-regulated ones. The GO analysis indicated that genes have many important functions such as metabolic metabolites, immune and inflammatory response. The pathway analysis highlighted the enhanced activity of staphylococcus aureus infection, chemokine and metabolic signaling pathways as well as cytokine-cytokine receptor interaction. 13 DEGs that verified by the quantitative real-time PCR were identified as key genes.
Compare to their healthy HLA-B27-positive blood relatives',monocytes from HLA-B27 associated AUU patients were more sensitive and had a greater inflammatory response to LPS stimulation. These characterized DEGs may play critical roles in the pathogenesis of AAU and could help provide new therapeutic ideas.
This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.
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