July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Expression of cytokines in porcine iris, retina and choroid tissues stimulated by microbe-associated molecular patterns
Author Affiliations & Notes
  • Yong Seop Han
    Casey Eye Institute, Oregon Health and Science University, Portland, Oregon, United States
    Ophthalmology, Gyeongsang National University, Jinju, Korea (the Republic of)
  • Erick Rivera-Grana
    Casey Eye Institute, Oregon Health and Science University, Portland, Oregon, United States
  • James T. Rosenbaum
    Casey Eye Institute, Oregon Health and Science University, Portland, Oregon, United States
    Legacy Devers Eye Institute, Portland, Oregon, United States
  • Matthew Schleisman
    Casey Eye Institute, Oregon Health and Science University, Portland, Oregon, United States
  • Sean Davin
    Casey Eye Institute, Oregon Health and Science University, Portland, Oregon, United States
  • Naman Jain
    Division of Arthritis and Rheumatology, Oregon Health and Science University, Portland, Oregon, United States
  • Melanie Alvarado
    Division of Arthritis and Rheumatology, Oregon Health and Science University, Portland, Oregon, United States
  • Mark Asquith
    Division of Arthritis and Rheumatology, Oregon Health and Science University, Portland, Oregon, United States
  • Footnotes
    Commercial Relationships   Yong Seop Han, None; Erick Rivera-Grana, None; James Rosenbaum, None; Matthew Schleisman, None; Sean Davin, None; Naman Jain, None; Melanie Alvarado, None; Mark Asquith, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 2526. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Yong Seop Han, Erick Rivera-Grana, James T. Rosenbaum, Matthew Schleisman, Sean Davin, Naman Jain, Melanie Alvarado, Mark Asquith; Expression of cytokines in porcine iris, retina and choroid tissues stimulated by microbe-associated molecular patterns. Invest. Ophthalmol. Vis. Sci. 2018;59(9):2526.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : The gut microbiota has been associated with immune-mediated uveitis in several studies. Mounting evidence indicates that translocation of microbes or microbial products from the gut may promote inflammation in the eye. We hypothesize that each tissue in the eye has a different composition of pattern recognition receptors (PRR) which might explain the variety of clinical features of immune-mediated uveitis. For this reason we investigated the PRR composition on porcine iris, retina and choroid. In addition, we sought to demonstrate the expression of cytokines in porcine iris, retina and choroid tissues stimulated by microbe-associated molecular patterns (MAMPs).

Methods : Freshly enucleated porcine eyes were dissected and tissues including iris, retina and choroid were biopsied. Tissues were subsequently stimulated overnight with microbial or fungal MAMPs (LPS, TL8-506, NG-MDP, FLA-ST or Curdlan). Cytokine (IL-6, IL-8, IL-1β, IL-12 and IL-23) mRNA expression was subsequently determined by qRT-PCR The expression of PRRs Dectin-1, DC-SIGN, TLR4, TLR8 and NOD2; and Dectin-1 signaling component Syk was also determined in these tissues by qRT-PCR. Relative expression of mRNA was normalized to housekeeping gene HPRT and compared to unstimulated controls.

Results : We found that expression of PRRs and Syk was present in all tissues, although substantially lower in retina compared to iris and choroid. MAMP-induced cytokine expression was also lowest in retina. The most strongly induced cytokines following MAMP stimulation were IL-1β, IL-6, and strikingly IL-23 with a minimal IL-12 response. Interestingly both microbial and particularly fungal (Curdlan) MAMPs elicited potent cytokine responses in both iris and choroid.

Conclusions : Retina, choroid and iris each express PRRs although with varying expression by tissue type. This was also paralleled by differential responses to MAMP-stimulation. None of these tissues was completely refractory to PRR stimulation, although diminished responses were observed in retina. Interestingly, a wide-range of microbial and fungal MAMPs were found to elicit inflammatory response in ocular tissues. Of these, Curdlan-induced IL-23 expression may be particularly relevant to the Th17 signature response known to accompany uveitis.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×