July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
The hierarchy of pro-inflammatory cytokines in ocular inflammation
Author Affiliations & Notes
  • Xiaoqiu Wu
    Ophthalmology, Novartis, Cambridge, Massachusetts, United States
  • Andre Cunha
    Ophthalmology, Novartis, Cambridge, Massachusetts, United States
  • Qin Zhang
    Ophthalmology, Novartis, Cambridge, Massachusetts, United States
  • Melissa Prentiss
    Ophthalmology, Novartis, Cambridge, Massachusetts, United States
  • Vanessa Kainz
    Ophthalmology, Novartis, Cambridge, Massachusetts, United States
  • YongYao Xu
    Ophthalmology, Novartis, Cambridge, Massachusetts, United States
  • Joanna Vrouvlianis
    Ophthalmology, Novartis, Cambridge, Massachusetts, United States
  • Hui Li
    Ophthalmology, Novartis, Cambridge, Massachusetts, United States
  • Nalini Rangaswamy
    Ophthalmology, Novartis, Cambridge, Massachusetts, United States
  • Barrett Leehy
    Ophthalmology, Novartis, Cambridge, Massachusetts, United States
  • Terri McGee
    Ophthalmology, Novartis, Cambridge, Massachusetts, United States
  • Christie Bell
    Ophthalmology, Novartis, Cambridge, Massachusetts, United States
  • Chad E Bigelow
    Ophthalmology, Novartis, Cambridge, Massachusetts, United States
  • Viral Kansara
    Ophthalmology, Novartis, Cambridge, Massachusetts, United States
  • Qian Huang
    Ophthalmology, Novartis, Cambridge, Massachusetts, United States
  • Henry Wu
    Ophthalmology, Novartis, Cambridge, Massachusetts, United States
  • Footnotes
    Commercial Relationships   Xiaoqiu Wu, None; Andre Cunha, None; Qin Zhang, None; Melissa Prentiss, None; Vanessa Kainz, None; YongYao Xu, None; Joanna Vrouvlianis, None; Hui Li, None; Nalini Rangaswamy, None; Barrett Leehy, None; Terri McGee, None; Christie Bell, None; Chad Bigelow, None; Viral Kansara, None; Qian Huang, None; Henry Wu, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 2532. doi:
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      Xiaoqiu Wu, Andre Cunha, Qin Zhang, Melissa Prentiss, Vanessa Kainz, YongYao Xu, Joanna Vrouvlianis, Hui Li, Nalini Rangaswamy, Barrett Leehy, Terri McGee, Christie Bell, Chad E Bigelow, Viral Kansara, Qian Huang, Henry Wu; The hierarchy of pro-inflammatory cytokines in ocular inflammation. Invest. Ophthalmol. Vis. Sci. 2018;59(9):2532.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : The concept of tissue dependent cytokine hierarchy has been demonstrated in a number of diseases but it has not been investigated in ophthalmic diseases. Here we evaluated the functional hierarchy of IL-1b, IL-6, IL-17A and TNF in the induction of ocular inflammation.

Methods : We delivered AAV vectors expressing IL-1β, IL-6, IL-17A, or TNF intravitreally in naïve C57/BL6 mice and compared and contrasted the inflammatory effects in the eye 5 weeks after AAV-mediated gene transfer. We also used an in vitro human system to test the effect of cytokines on barrier function.

Results : We found that IL-1b had the highest ability to initiate ocular inflammation. The continuous overexpression of IL-1b resulted in a significant upregulation of additional pro-inflammatory mediators in the eye. Using SLO and OCT imaging techniques we showed that a low dose of AAVIL-1β was sufficient and was as pathogenic as a high dose of TNF in inducing vascular leakage, retinal degeneration and cellular infiltration. Furthermore, only a marginal increase in IL-1b was enough to cause cellular infiltration thus confirming the highly pathogenic nature of IL-1b in the eye. Contrary to our expectation, IL-6 or IL-17A had minimal or no effect in the eye. To examine the clinical relevance of our findings we used an impedance assay to show that IL-1β alone or TNF alone was able to cause primary human retinal endothelial cell barrier dysfunction in vitro. Again, IL-6 alone or IL-17A alone had no effect on barrier function however, in the presence of IL-1b or TNF, IL-17A but not IL-6 may provide additive pro-inflammatory effects.

Conclusions : Our studies demonstrate the existence of a functional hierarchy of pro-inflammatory cytokines in the eye and we show that IL-1b is the most pathogenic when it is continuously expressed in the eye.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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