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Shuo Yu, Xinli Liu, Nan Zhang, Cui Mao, Hong Lu; Gene expression analysis of Hedysarum polybotrys saccharide(HPS) in Endotoxin induced acute anterior uveitis. Invest. Ophthalmol. Vis. Sci. 2018;59(9):2533.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the key genes and the inflammatory signaling pathway involved in the anti-inflammatory effect of HPS on rat model of EIU.
EIU in Wistar rats was developed by subcutaneous injection of LPS (200 μg). HPS (400mg/kg, intraperitoneally) or its carrier was injected 24h and 1h before EIU induction. 24 hours after EIU, eyes were examined then enucleated. Total RNA was extract from iris-ciliary body. Gene expression microarrays were used to identify the differentially expressed genes (DEGs), and the DEGs were analyzed by a range of bioinformatics-based techniques including Gene Ontology (GO), Pathway analysis, and Gene Correlation Network.
The gene expression microarrays identified 322 DEGs, including 254 mRNA genes and 68 lncRNA genes. The GO analysis reflected many important functions, and the pathway analysis also highlighted several signaling pathways. 16 mRNA and 7 lncRNA DEGs that were shown to play critical roles in the Gene Correlation Network.
A primarily combined mRNA and lncRNA gene expression analysis of HPS targets on EIU displayed a series of candidate genes, which indicated that the anti-inflammatory effect of HPS relied on combined signal transduction pathways in vivo.
This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.
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