July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Development of cornea organoids from human induced pluripotent stem cells and their future use
Author Affiliations & Notes
  • Shukti Chakravarti
    Medicine, Johns Hopkins School of Medicine, Baltimore, Maryland, United States
    Ophthalmology, Johns Hopkins School of Medicine, Baltimore, Maryland, United States
  • Footnotes
    Commercial Relationships   Shukti Chakravarti, None
  • Footnotes
    Support  NIH grant EY026104
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 2610. doi:https://doi.org/
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      Shukti Chakravarti; Development of cornea organoids from human induced pluripotent stem cells and their future use. Invest. Ophthalmol. Vis. Sci. 2018;59(9):2610. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Presentation Description : Tissue organoids have become powerful tools to probe specific cellular functions, interactions and underlying mechanisms in health and disease. Human iPS cells grown under low attachment, hypoxic conditions were treated to differentiation programs that support neural induction. A fraction of the developing optic vesicles lacked pigmented cells and developed into translucent organoids. We characterized these organoids and found these to harbor key features of the multi-layered cornea. Thus, the cornea-organoids expressed epithelial markers, KRT14, KRT3, p63, stromal markers, lumican, keratocan, collagen types I and V, and endothelial collagen type VIII. Most importantly, by TEM we detected collagen fibrils with characteristic organization and stacking that suggest expansion of a corneal stromal matrix. These cornea organoids will be used to study matrix assembly, cell-matrix interactions and the effects of specific genetic alterations on these processes.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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