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Rong Wen, Peiyu Li, Zhijun Luo, Yiwen Li; Inhibition of CNV (choroidal neovascularization) by berberine. Invest. Ophthalmol. Vis. Sci. 2018;59(9):2633.
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© ARVO (1962-2015); The Authors (2016-present)
Berberine is a benzylisoquinoline alkaloid found in a variety of medicinal herbs with anti-angiogenesis properties. The present work examines the potential of berberine in inhibiting CNV using the Matrigel CNV rat model.
CNV was induced in adult female Sprague-Dawley rats by subretinal injection of Matrigel. Berberine powder was added to PBS (10 µg/µl) as a suspension and mixed with Matrigel at a 1:3 ratio immediately before injecting to the right eyes (1.2 µl/eye). The left eyes were injected with Matrigel diluted at 3:1 with PBS only. Animals were killed 10 days later and perfused with a DiI solution to stain blood vessels. Eyes were collected and embedded in 5% agarose. Serial sections were cut on a vibratome to cover the entire Matrigel area and examined by confocol microscopy. CNV area was calculated and analyzed.
The average CNV affected area in the in the control group is calculated to be 293.97±149.60 (x 1,000 µm2, mean±SD, n=7). The berberine treated eyes, on the other hand, had an average CNV area of 87.41±70.43 (x 1,000 µm2, mean±SD, n=8), significantly smaller than the control eyes (P<0.01).
Berberine significantly inhibits CNV in the subretinal Matrigel CNV model.
This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.
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