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Sang Hoon Jung, Kyung-A Kim, Kui Dong Kang, Su Ah Kim, Tae-Jin Kim; The effects of curcumin on Src activity in live ARPE-19 cells visualized by FRET. Invest. Ophthalmol. Vis. Sci. 2018;59(9):3069.
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© ARVO (1962-2015); The Authors (2016-present)
The purpose of this study is to investigate the dynamics of Src activity in response to curcumin in human retinal pigment epithelial cells (ARPE-19).
ARPE-19 cells transfected with genetically encoded Src biosensors based on fluorescence resonance energy transfer (FRET) were followed by the incubation with either normal condition (5.5 mM glucose) or hyperglycemia (22 mM glucose) for 24 hr. After pretreatment of curcumin (1 uM), Src activity was monitored by confocal microscopy. The FRET measurement was performed by quantifying cyan fluorescent protein (CFP) and yellow fluorescent protein (YFP) ratios using Leica Application Suite 2.02 software.
Our results revealed that curcumin significantly increased the FRET ratio of Src biosensors in ARPE-19 cells under normal condition. Src signals of non-lipid rafts membrane were activated earlier than that of the lipid rafts membrane, whereas reduced Src activities were observed under hyperglycemia condition after curcumin treatment.
These findings suggest that curcumin-induced Src activity was correlated with glucose metabolism in ARPE-19 cells. It may provide valuable information for understanding the underlying mechanism of Src regulation and its pathophysiological role on diabetic retinopathy.
This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.
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