July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Overproduction ß-amyloid peptides and overactivity of the amyloidogenic pathaway in retina, optic nerve and visual cortex in healthy aging and Alzheimer s disease
Author Affiliations & Notes
  • LUIS F HERNANDEZ
    Research, Asociación Para Evitar la Ceguera en México, Mexico, Mexico, Mexico
  • Montserrat Perez
    Research, Asociación Para Evitar la Ceguera en México, Mexico, Mexico, Mexico
  • Roberto Gonzalez-Salinas
    Research, Asociación Para Evitar la Ceguera en México, Mexico, Mexico, Mexico
  • Rosario Gulias-Cañizo
    Research, Asociación Para Evitar la Ceguera en México, Mexico, Mexico, Mexico
  • Lenin Ochoa-de la Paz
    Research, Asociación Para Evitar la Ceguera en México, Mexico, Mexico, Mexico
  • Hugo Quiroz-Mercado
    Research, Asociación Para Evitar la Ceguera en México, Mexico, Mexico, Mexico
  • Rubén Zamora-Alvarado
    Research, Asociación Para Evitar la Ceguera en México, Mexico, Mexico, Mexico
  • Footnotes
    Commercial Relationships   LUIS F HERNANDEZ, None; Montserrat Perez, None; Roberto Gonzalez-Salinas, None; Rosario Gulias-Cañizo, None; Lenin Ochoa-de la Paz, None; Hugo Quiroz-Mercado, None; Rubén Zamora-Alvarado, None
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 3070. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      LUIS F HERNANDEZ, Montserrat Perez, Roberto Gonzalez-Salinas, Rosario Gulias-Cañizo, Lenin Ochoa-de la Paz, Hugo Quiroz-Mercado, Rubén Zamora-Alvarado; Overproduction ß-amyloid peptides and overactivity of the amyloidogenic pathaway in retina, optic nerve and visual cortex in healthy aging and Alzheimer s disease. Invest. Ophthalmol. Vis. Sci. 2018;59(9):3070.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : To evaluate changes in the production of beta amyloid peptides and the expression of the enzymes involved in the production of these peptides in the following areas; visual cortex, optic nerve and retina in healthy aging (C57/BL6 mice, 4 months old versus 18 Months old) and wild type mice versus AD triple transgenic mice (3xTg-AD).

Methods : Double IF assays on coronary slides (10µm) of visual cortex, retina and optic nerve of young mice (4M) and old mice (25M), as well as wild type mice (4M) and 18M of 3xTg-AD were performed. Monoclonal antibodies for each one of the enzymes involved were used ; non amyloidogenic (alfa-secretase); ADAM10, ADAM9; Amyloidogenic pathway; BACE, Preseniline2 (PRES2) (The principal enzyme involved in ßA42 peptide production). Finally, to verify the expression changes of these enzymes, qRT-PCR assays for each enzyme were performed using mRNA of the same anatomic regions of the animals used.

Results : There is an significant expression increase of the enzymes, BACE, ADAM 9, PRES2 and a reduction of ADAM 10 in retina, O.N. and VC on aged mice as well as on 3xTg-AD mice.

Conclusions : 1) These models are useful to study the production of amyloid beta in these anatomical areas in aging and in Alzheimer s disease. 2) The increase of the enzymes involved in the amyloidogenic pathway in the retina, O.N. and VC, suggest that ßA42 accumulation on these regions is a consequence of the over activation of this pathway in normal aging and Alzheimer s Disease. 3) These changes could be associated to the visual loss function observed in AD patients.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×