July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Sulfamethazine impairs retinal function by interruption of microRNA activity
Author Affiliations & Notes
  • Xue-Jiao Chen
    Wenzhou Medical University, Division of Ophthalmic Genetics, Lab for Stem Cell & Retinal Regeneration, Wenzhou, China
  • Kun-Chao Wu
    Wenzhou Medical University, Division of Ophthalmic Genetics, Lab for Stem Cell & Retinal Regeneration, Wenzhou, China
  • Chang-Jun Zhang
    Wenzhou Medical University, Division of Ophthalmic Genetics, Lab for Stem Cell & Retinal Regeneration, Wenzhou, China
  • Zi-Bing Jin
    Wenzhou Medical University, Division of Ophthalmic Genetics, Lab for Stem Cell & Retinal Regeneration, Wenzhou, China
  • Footnotes
    Commercial Relationships   Xue-Jiao Chen, None; Kun-Chao Wu, None; Chang-Jun Zhang, None; Zi-Bing Jin, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 3072. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Xue-Jiao Chen, Kun-Chao Wu, Chang-Jun Zhang, Zi-Bing Jin; Sulfamethazine impairs retinal function by interruption of microRNA activity. Invest. Ophthalmol. Vis. Sci. 2018;59(9):3072.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Antibiotics has made an epoch in the history for fighting against bacterial infections in human and has been commonly used in livestock to treat animal infections and improve production. However, the studies of some antibiotics on nervous system in mammals are rare and the underlying mechanism is poorly understood.

Methods : Electroretinogram (ERG) was carried out to determine the retinal function of mammals. Fundus photographs, Optical coherence tomography (OCT), immunostaining and electron microscopy were performed to investigate the retinal structure. QPCR and western blotting were conducted to detect related genes expression. Luciferase reporter assay validated the sulfamethazine on miRNA function. The relationship between sulfamethazine and target protein were revealed through Auto duck software and SPR in vitro.

Results : Herein we show that a short-term oral administration of veterinary antibiotic sulfamethazine (SM2) would lead to significant residue in retina and thereafter retinal dysfunction in mice, guinea pig and non-human primate. Furthermore, we observe that pregnant mice intaking SM2 results in apparent retinal damage in the offsprings. We demonstrate that SM2 interrupt miRNA activity through binding to Ago2 directly in vitro, which was further confirmed by Surface plasmon resonance (SPR). Using AAV mediated si-Ago2 in mice retina, we find Ago2 is indispensable for the maintenance of retinal structure and function.

Conclusions : Our results discover that SM2 endanger retinal function through targeting on Ago2, implicating a potential side effect of residual sulfamethazine in human dietary meat derived from food-producing animals worldwide.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×