Abstract
Purpose :
Development of LC-MS method for the quantification of ocular bisretinoids and sequential analysis of their levels in age matched healthy cadaver eye.
Methods :
Cadaver donor from below 40 age group (n=42), 40-60 age group (n=78) and 60-80 age group (n=80) were obtained after the study protocol was approved by the standing Institutional Review Board of Aravind Eye Hospital, Madurai (IRB #.RES2011002BAS) and subsequently by human ethics board of All India Institute of Medical Sciences, New Delhi (IRB # IESC/T-347/23.06.2015). Macular RPE was obtained by using a 8mm punch and RPE from periphery was collected 2mm away from the macular sample. Samples were quantified based on relative quantification method developed using LC-ESI-MS.
Results :
The levels of A2GPE were observed to be 0.48 ± 0.04(macula) and 1.48 ± 0.14(periphery) extract/ml of below 40 age group, 0.48 ± 0.04(macula) and 2.27 and 0.14 (periphery) extract/ml in 40-60 age group and 0.36 ± 0.02(macula) and 2.24 ± 0.18 (periphery) in 60-80 age group. The levels of A2DHPE were observed to be 0.04 ± 0.06(macula) and 0.13 ± 0.12(periphery) extract/ml in below 40 age group, 0.08 ± 0.11(macula) and 0.47 ± 0.63(periphery) extract/ml in 40-60 years and 0.10 ± 0.09(macula) and 1.38 ± 1.60 (periphery) extract/ml in 60-80 years. The levels of MFA2E were observed to be 0.32 ± 0.05 (macula) and 0.89 ± 0.13 (periphery) extract/ml of below 40 age group, 0.39 ± 0.05(macula) and 2.20 ± 0.17(periphery) extract/ml in 40-60 years age group and 0.52± 0.06 (macula) and 2.7 ± 0.19 (periphery) in 60-80 years of age.
Conclusions :
Increase trend observed for A2GPE, A2DHPE and MFA2E with age in peripheral retina and a significant increase observed from macula to peripheral retina in normal cadaver eyes.
This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.