July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Evaluation of the protective effect of sodium thiosulfate (STS) on a Dry Eye Disease experimental model on C57BL / 6J mice
Author Affiliations & Notes
  • Roberto Gonzalez-Salinas
    Research Department, Asociación para Evitar la Ceguera en México, Mexico City, Mexico
  • Luis F Hernández-Zimbrón
    Research Department, Asociación para Evitar la Ceguera en México, Mexico City, Mexico
  • Rosario Gulias-Cañizo
    Research Department, Asociación para Evitar la Ceguera en México, Mexico City, Mexico
  • Lenin Ochoa-de-la-Paz
    Research Department, Asociación para Evitar la Ceguera en México, Mexico City, Mexico
  • Rubén Zamora-Alvarado
    Research Department, Asociación para Evitar la Ceguera en México, Mexico City, Mexico
  • Fátima Rubio-Tijerina
    Anterior Segment Surgery Department, Asociación para Evitar la Ceguera en México, Mexico City, Mexico
  • Hugo Quiroz-Mercado
    Research Department, Asociación para Evitar la Ceguera en México, Mexico City, Mexico
  • Footnotes
    Commercial Relationships   Roberto Gonzalez-Salinas, None; Luis Hernández-Zimbrón, None; Rosario Gulias-Cañizo, None; Lenin Ochoa-de-la-Paz, None; Rubén Zamora-Alvarado, None; Fátima Rubio-Tijerina, None; Hugo Quiroz-Mercado, DigiSight Technologies Inc (C)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 3280. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Roberto Gonzalez-Salinas, Luis F Hernández-Zimbrón, Rosario Gulias-Cañizo, Lenin Ochoa-de-la-Paz, Rubén Zamora-Alvarado, Fátima Rubio-Tijerina, Hugo Quiroz-Mercado; Evaluation of the protective effect of sodium thiosulfate (STS) on a Dry Eye Disease experimental model on C57BL / 6J mice
      . Invest. Ophthalmol. Vis. Sci. 2018;59(9):3280.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Sodium thiosulphate (STS) have been shown to reduce the expression of molecules associated to inflammation in corneal endothelium caused by systemic Lipopolysaccharide administration in mice. Consequently, STS could be employed as an adjuvant anti-inflammatory therapy for several pathological conditions associated with inflammation in the eye. In the present study, we evaluated the effects of STS on a Dry Eye Disease (DED) experimental model on young C57BL/6J mice.

Methods : Chronic DED was induced by exposing C57BL/6 mice to desiccating stress using a controlled environment chamber for 12 days.
Animals
Young (2-month-old) female C57BL/6J mice were maintained on a 12h light/dark cycle in a temperature controlled room. Animal handling was approved by the Institutional Animal Care and Use Committee, which adheres to the national law and NIH rules.
Experimental design
Twelve mice (three mice per group) were used in each experiment in three experimental conditions: Saline (SSB) only group without desiccating stress (control), desiccating stress without vehicle, vehicle+desiccating stress and vehicle+desiccating stress+STS(750mg/kg). Three independent experiments were performed.
Double immunofluorescence assays
Eye tissue obtained from both eyes of each mice was postfixed in paraformaldehyde 4% during 24 hr and embedded in paraffine (Mc Cormick, St. Louis, MO, USA). 10 μm thick corneal sections were cut using a microtome (American Optical) and mounted on slides. Corneal slices from each group were processed and stored until use for immunohistochemistry for IL-1β, IL-6, TNF-alpha, GFAP and TSPO.
Data were analyzed using ANOVA and post-hoc Tukey’s test for multiple comparisons. Differences between groups were considered statistically significant when P < 0.05. Analyses were performed with Prism GraphPad Software v.6

Results : STS reduced corneal levels of pro-inflammatory cytokine interleukin-1β (IL-1β), IL-6, TNF-alpha, Glial acidic fibrillary protein (GFAP) and 18 kDa translocator protein (TSPO) in corneal epithelium in DED experimental model.

Conclusions : To the best of our knowledge, this is the first report describing the corneal anti-inflammatory effect of STS in a Dry Eye Disease experimental model on young C57BL/6J mice. Our results suggest the potential new use for STS, an already approved drug for human use.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×