July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Unilateral corneal nerve cutting induces bilateral dry eye disease in a murine model.
Author Affiliations & Notes
  • JinSuk Ryu
    Department of Ophthalmology, Seoul National University Hospital, Seoul , Korea (the Republic of)
    Laboratory of Ocular Regenerative Medicine and Immunology, Biomedical Research Institute, Seoul National University Hospital, Seoul, Korea (the Republic of)
  • Hyo Kyung Lee
    Department of Ophthalmology, Seoul National University Hospital, Seoul , Korea (the Republic of)
    Laboratory of Ocular Regenerative Medicine and Immunology, Biomedical Research Institute, Seoul National University Hospital, Seoul, Korea (the Republic of)
  • Hyun Jeong Jeong
    Department of Ophthalmology, Seoul National University Hospital, Seoul , Korea (the Republic of)
    Laboratory of Ocular Regenerative Medicine and Immunology, Biomedical Research Institute, Seoul National University Hospital, Seoul, Korea (the Republic of)
  • Chang Ho Yoon
    Department of Ophthalmology, Seoul National University Hospital, Seoul , Korea (the Republic of)
    Laboratory of Ocular Regenerative Medicine and Immunology, Biomedical Research Institute, Seoul National University Hospital, Seoul, Korea (the Republic of)
  • Mee Kum Kim
    Department of Ophthalmology, Seoul National University Hospital, Seoul , Korea (the Republic of)
    Laboratory of Ocular Regenerative Medicine and Immunology, Biomedical Research Institute, Seoul National University Hospital, Seoul, Korea (the Republic of)
  • Footnotes
    Commercial Relationships   JinSuk Ryu, None; Hyo Kyung Lee, None; Hyun Jeong Jeong, None; Chang Ho Yoon, None; Mee Kum Kim, None
  • Footnotes
    Support  This study was supported by a grant from Seoul National University Hospital Research Fund (Project No.: 04-2017-0260).
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 3290. doi:https://doi.org/
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      JinSuk Ryu, Hyo Kyung Lee, Hyun Jeong Jeong, Chang Ho Yoon, Mee Kum Kim; Unilateral corneal nerve cutting induces bilateral dry eye disease in a murine model.. Invest. Ophthalmol. Vis. Sci. 2018;59(9):3290. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To investigate the effect of the severing of corneal basal nerve plexus in one eye on bilateral dry eye disease in a murine model.

Methods : 6 week-old female BALB/c mice were divided into control (n=30) and nerve-cutting (NC) groups (n=30). The left cornea was partially incised with a 2.0mm circular trephine, through the upper two-thirds of the cornea stromal layer. Lissamine green corneal staining and tear volume measurements were performed every week for 4 weeks. At 2 and 4 weeks, dendritic cells, CD4+/CD8+ T cells, regulatory T cells in cervical lymph nodes, conjunctivocornea and spleens were analyzed by flow cytometry. With real-time RT-PCR, neuropeptides were also analyzed in intra-orbital lacrimal glands at 2 weeks.

Results : In bilateral eyes of mice in NC group, corneal staining grades were increased and tear volumes were decreased. CD11c+CD86hi, CD11c+MHCIIhi cells in conjunctivocornea were increased in trephined eyes at 2 weeks and in contralateral eyes at 4 weeks. CD69 were highly expressed in CD4+/CD8+ T cells in conjunctivocornea of trephined eyes at 4 weeks. CD8+IFNγ+ T cells were increased in contralateral cervical lymph nodes in NC group. Meanwhile, regulatory T cells in spleens were significantly suppressed in NC group. In addition, neuropeptide Y and neutrin were increased in intra-orbital lacrimal glands in NC group.

Conclusions : It suggests that unilateral corneal nerve cutting may induce bilateral dry eye disease by entering activated dendritic cells into the bilateral eyes with a disturbance of immune privilege.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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