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JinSuk Ryu, Hyo Kyung Lee, Hyun Jeong Jeong, Chang Ho Yoon, Mee Kum Kim; Unilateral corneal nerve cutting induces bilateral dry eye disease in a murine model.. Invest. Ophthalmol. Vis. Sci. 2018;59(9):3290.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the effect of the severing of corneal basal nerve plexus in one eye on bilateral dry eye disease in a murine model.
6 week-old female BALB/c mice were divided into control (n=30) and nerve-cutting (NC) groups (n=30). The left cornea was partially incised with a 2.0mm circular trephine, through the upper two-thirds of the cornea stromal layer. Lissamine green corneal staining and tear volume measurements were performed every week for 4 weeks. At 2 and 4 weeks, dendritic cells, CD4+/CD8+ T cells, regulatory T cells in cervical lymph nodes, conjunctivocornea and spleens were analyzed by flow cytometry. With real-time RT-PCR, neuropeptides were also analyzed in intra-orbital lacrimal glands at 2 weeks.
In bilateral eyes of mice in NC group, corneal staining grades were increased and tear volumes were decreased. CD11c+CD86hi, CD11c+MHCIIhi cells in conjunctivocornea were increased in trephined eyes at 2 weeks and in contralateral eyes at 4 weeks. CD69 were highly expressed in CD4+/CD8+ T cells in conjunctivocornea of trephined eyes at 4 weeks. CD8+IFNγ+ T cells were increased in contralateral cervical lymph nodes in NC group. Meanwhile, regulatory T cells in spleens were significantly suppressed in NC group. In addition, neuropeptide Y and neutrin were increased in intra-orbital lacrimal glands in NC group.
It suggests that unilateral corneal nerve cutting may induce bilateral dry eye disease by entering activated dendritic cells into the bilateral eyes with a disturbance of immune privilege.
This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.
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