Abstract
Purpose :
To investigate the effect of anti-CD80/86 antibody on CD4+CD25+Foxp3+ regulatory T cells (Tregs) at ipsilateral draining lymph nodes (dLNs) in murine corneal transplantation.
Methods :
Corneal grafts from C57BL6 mice were transplanted to BALB/c mice. Anti-CD80/86 (50 μg) was administered to transplanted mice by intraperitoneal injection (i.p.) every 24 hours post transplantation for 3 days (IP3 group), 5 days (IP5 group) or 7 days (IP7 group); PBS was administered as control. dLNs were harvested 7 days after transplantation and analyzed for Tregs, CD11c+ MHC class II + dendritic cells (mDC) by flow cytometry. The expression level of Foxp3, CTLA-4 in Tregs and MHC2 in dendritic cells were analyzed by mean fluorescence intensity (MFI) using flow cytometry.
Results :
There were no significant differences between the frequencies of CD4+ cells among groups. Anti-CD80/86 administration reduced the frequencies of Tregs compared to control (control:13.3%, IP3:8.8%, IP5:7.8％, IP7:8.0%, p<0.0001). After anti-CD80/86 administration, there was an increase in MFI expression of CTLA-4 in dLN compared to control, but no differences were noted for Foxp3 (Foxp3; control:10401, IP3:11171, IP5:9856, IP7:9478, p<0.07, CTLA-4; control: 3723, IP3: 2359, IP5: 2050, IP7: 1996, p<0.001). There were no differences in frequencies of mDC and the MFI expression of MHC class II after anti-CD80/86 administration.
Conclusions :
Anti-CD80/86 administration for more than 3 days causes differentiation failure of Tregs in dLNs.
This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.