July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Rho-Kinase Inhibitor Ripasudil suppresses dendritic cell maturation in murine corneal transplantation
Author Affiliations & Notes
  • maria miura
    Juntendo University, Tokyo, Japan
  • Takenori Inomata
    Juntendo University, Tokyo, Japan
  • Mikiko Okano
    Juntendo University, Tokyo, Japan
  • Keiichi Fujimoto
    Juntendo University, Tokyo, Japan
  • Yuichi Okumura
    Juntendo University, Tokyo, Japan
  • Tina Shiang
    Orange Park Medical Center, Jacksonville, Florida, United States
  • Toshinari Funaki
    Juntendo University, Tokyo, Japan
  • Akira Murakami
    Juntendo University, Tokyo, Japan
  • Footnotes
    Commercial Relationships   maria miura, None; Takenori Inomata, KOWA Company. Ltd. (F); Mikiko Okano, None; Keiichi Fujimoto, None; Yuichi Okumura, None; Tina Shiang, None; Toshinari Funaki, KOWA Company. Ltd. (F); Akira Murakami, KOWA Company. Ltd. (F)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 3308. doi:
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      maria miura, Takenori Inomata, Mikiko Okano, Keiichi Fujimoto, Yuichi Okumura, Tina Shiang, Toshinari Funaki, Akira Murakami; Rho-Kinase Inhibitor Ripasudil suppresses dendritic cell maturation in murine corneal transplantation. Invest. Ophthalmol. Vis. Sci. 2018;59(9):3308.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose : To assess the immunological effects of Rho-Kinase Inhibitor (Ripasudil) on murine corneal transplantation.

Methods : Donor corneas (C57BL6) were transplanted onto BALB/c recipient mice. Placebo instillation (P group), 0.04% Ripasudil (R1 group), 0.4% Ripasudil (R2 group), 2.0% Ripasudil (R3 group) were instilled three times a day post transplantation immediately. Draining lymph nodes (dLNs) were harvested at day 14 post-transplantation (n=5 mice/group). We analyzed the frequencies of CD4+CD25+Foxp3+regulatory T cells and MHCIIhiCD11c+ mature dendritic cells (DCs) in the host dLNs using flow cytometry. The expression of Foxp3, CTLA-4 on Treg and MHCII of DCs were analyzed by mean fluorescence intensity (MFI).

Results : Treg was significantly decreased in the R3 group compared to the P group (14.95% in P group, 14.77% in R1 group, 15.22% in R2 group, 13.60% in R3 group, p = 0.0096). MHCIIhiCD11c+ mature DCs were significantly decreased in the R2 and R3 groups compared to the P group (63.22% in P group, 59.52% in R1 group, 56.78% in R2 group, 55.12% in R3 group, p = 0.003). The MFI expression of Foxp3 in Tregs showed a significant decrease in the R3 group (P: 2398, R1: 2332, R2: 2347, R3: 2165, p = 0.0243) and the expression of MHC2 in DCs showed a significant decrease in the R3 group (P: 10889, R1: 10142, R2: 10034, R3: 7666, p = 0.0023). There was no significant difference in the expression of CTLA-4 in each group.

Conclusions : Ripasudil instillation could inhibit maturation of dendritic cells in corneal transplantation.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.


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