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maria miura, Takenori Inomata, Mikiko Okano, Keiichi Fujimoto, Yuichi Okumura, Tina Shiang, Toshinari Funaki, Akira Murakami; Rho-Kinase Inhibitor Ripasudil suppresses dendritic cell maturation in murine corneal transplantation. Invest. Ophthalmol. Vis. Sci. 2018;59(9):3308. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
To assess the immunological effects of Rho-Kinase Inhibitor (Ripasudil) on murine corneal transplantation.
Donor corneas (C57BL6) were transplanted onto BALB/c recipient mice. Placebo instillation (P group), 0.04% Ripasudil (R1 group), 0.4% Ripasudil (R2 group), 2.0% Ripasudil (R3 group) were instilled three times a day post transplantation immediately. Draining lymph nodes (dLNs) were harvested at day 14 post-transplantation (n=5 mice/group). We analyzed the frequencies of CD4+CD25+Foxp3+regulatory T cells and MHCIIhiCD11c+ mature dendritic cells (DCs) in the host dLNs using flow cytometry. The expression of Foxp3, CTLA-4 on Treg and MHCII of DCs were analyzed by mean fluorescence intensity (MFI).
Treg was significantly decreased in the R3 group compared to the P group (14.95% in P group, 14.77% in R1 group, 15.22% in R2 group, 13.60% in R3 group, p = 0.0096). MHCIIhiCD11c+ mature DCs were significantly decreased in the R2 and R3 groups compared to the P group (63.22% in P group, 59.52% in R1 group, 56.78% in R2 group, 55.12% in R3 group, p = 0.003). The MFI expression of Foxp3 in Tregs showed a significant decrease in the R3 group (P: 2398, R1: 2332, R2: 2347, R3: 2165, p = 0.0243) and the expression of MHC2 in DCs showed a significant decrease in the R3 group (P: 10889, R1: 10142, R2: 10034, R3: 7666, p = 0.0023). There was no significant difference in the expression of CTLA-4 in each group.
Ripasudil instillation could inhibit maturation of dendritic cells in corneal transplantation.
This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.
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