Purchase this article with an account.
Ryota Ko, Masahiko Hayashi, Miho Tanaka, Eiichi Uchio; Effects of Asian dust particles in a reconstructed cultured human corneal epithelial model. Invest. Ophthalmol. Vis. Sci. 2018;59(9):3324.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Asian Dust particles (ADP) are well known air pollutants in Japan. Asian dust originates from Northern China and spreads over China and some parts of Japan by wind. It is known as the major air pollutant exacerbating allergic diseases; however, the underlying mechanism is not yet clear. We evaluated the effects of ADPs on the viability of reconstructed cultured human corneal epithelial model (LabCyte CORNEA-MODEL).
The LabCyte CORNEA-MODEL was incubated with ADP (10–100 µg/mL) for 24 h. Cell viability was evaluated by the 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium,monosodium salt assay. Urban aerosol (UA) containing diesel exhaust particles was evaluated as a positive control. The results correspond to three independent experiments that were performed in triplicate. A one-way ANOVA test (α = 0.05) and Dunnett’s test (α = 0.05) were used for statistical analysis in SPSS 23.0 (Sun Microsystems, Inc., Santa Clara, CA). Hematoxylin and eosin staining (HE) was performed as pathological examination.
ADP and UA decreased the viability of the LabCyte CORNEA-MODEL in a dose-dependent manner. Pathological examination showed increase in thickness with elevated density.
ADP and UA affected viability and morphological changes, which suggested one of the factors showing exacerbated on corneal epithelium disorder and allergic diseases.
This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.
This PDF is available to Subscribers Only