July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Effects of Asian dust particles in a reconstructed cultured human corneal epithelial model
Author Affiliations & Notes
  • Ryota Ko
    Ophthalmology, Fukuoka University, Fukuoka-shi, Fukuoka, Japan
  • Masahiko Hayashi
    Earth System Science, Fukuoka University, Fukuoka-shi, Japan
  • Miho Tanaka
    Kobayashi Pharmaceutical Co., Ltd., Osaka-shi, Osaka, Japan
  • Eiichi Uchio
    Ophthalmology, Fukuoka University, Fukuoka-shi, Fukuoka, Japan
  • Footnotes
    Commercial Relationships   Ryota Ko, KOBAYASHI Pharmaceutical Co.,Ltd. (F); Masahiko Hayashi, Kobayashi Pharmaceutical Co., Ltd. (F); Miho Tanaka, None; Eiichi Uchio, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 3324. doi:
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    • Get Citation

      Ryota Ko, Masahiko Hayashi, Miho Tanaka, Eiichi Uchio; Effects of Asian dust particles in a reconstructed cultured human corneal epithelial model. Invest. Ophthalmol. Vis. Sci. 2018;59(9):3324.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Asian Dust particles (ADP) are well known air pollutants in Japan. Asian dust originates from Northern China and spreads over China and some parts of Japan by wind. It is known as the major air pollutant exacerbating allergic diseases; however, the underlying mechanism is not yet clear. We evaluated the effects of ADPs on the viability of reconstructed cultured human corneal epithelial model (LabCyte CORNEA-MODEL).

Methods : The LabCyte CORNEA-MODEL was incubated with ADP (10–100 µg/mL) for 24 h. Cell viability was evaluated by the 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium,monosodium salt assay. Urban aerosol (UA) containing diesel exhaust particles was evaluated as a positive control. The results correspond to three independent experiments that were performed in triplicate. A one-way ANOVA test (α = 0.05) and Dunnett’s test (α = 0.05) were used for statistical analysis in SPSS 23.0 (Sun Microsystems, Inc., Santa Clara, CA). Hematoxylin and eosin staining (HE) was performed as pathological examination.

Results : ADP and UA decreased the viability of the LabCyte CORNEA-MODEL in a dose-dependent manner. Pathological examination showed increase in thickness with elevated density.

Conclusions : ADP and UA affected viability and morphological changes, which suggested one of the factors showing exacerbated on corneal epithelium disorder and allergic diseases.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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