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Jiantao Wang; microRNA-1 regulates fibronectin expression in human trabecular meshwork cells under oxidative stress. Invest. Ophthalmol. Vis. Sci. 2018;59(9):3511.
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Fibronectin has been demonstrated to be involved in the pathogenesis of glaucoma, meanwhile, microRNAs (miRs) may play an important role in this disease. However, the relationship of miR-1 and fibronectin in glaucoma still keeps unclear. The purpose of this study was to investigate whether miR-1 could regulate fibronectin in glaucoma.
The expression levels of fibronectin mRNA and miR-1 in human trabecular meshwork cells (HTMCs) under oxidative stress were evaluated by quantitative polymerase chain reaction (qPCR). Luciferase reporter assay and bioinformatics were used to test the target of miR-1. The effects of miR-1 on the fibronectin were assessed by qPCR, western blot and immunofluorescence technique.
The mRNA level of fibronectin was increased, however, the expression level of miR-1 was downregulated in HTMCs under oxidative stress. MiR-1 targeted fibronectin through 3’-untranslated region (3’-UTR). Overexpression of miR-1 inhibited the expression of fibronectin in HTMCs under oxidative stress.
miR-1 could directly regulate the expression of fibronectin in HTMCs under oxidative stress, which may provide a novel therapeutic strategy in glaucoma.
This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.
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