July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Complement Factor 3 Profile in Aqueous Humour of Patients Across Primary Angle Closure Disease Spectrum
Author Affiliations & Notes
  • Monisha Nongpiur
    Singapore Eye Research Institute, Singapore, Singapore
    Singapore National Eye Centre, Singapore, Singapore
  • Lei Zhou
    Singapore Eye Research Institute, Singapore, Singapore
  • Siew Kwan Koh
    Singapore Eye Research Institute, Singapore, Singapore
  • Roger W. Beuerman
    Singapore Eye Research Institute, Singapore, Singapore
  • Eranga Vithana
    Singapore Eye Research Institute, Singapore, Singapore
  • Tin Aung
    Singapore Eye Research Institute, Singapore, Singapore
    Singapore National Eye Centre, Singapore, Singapore
  • Tina Wong
    Singapore Eye Research Institute, Singapore, Singapore
    Singapore National Eye Centre, Singapore, Singapore
  • Footnotes
    Commercial Relationships   Monisha Nongpiur, None; Lei Zhou, None; Siew Koh, None; Roger Beuerman, None; Eranga Vithana, None; Tin Aung, None; Tina Wong, None
  • Footnotes
    Support  National Medical Research Council, Singapore NMRC/TA/0047/2016
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 3518. doi:
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      Monisha Nongpiur, Lei Zhou, Siew Kwan Koh, Roger W. Beuerman, Eranga Vithana, Tin Aung, Tina Wong; Complement Factor 3 Profile in Aqueous Humour of Patients Across Primary Angle Closure Disease Spectrum. Invest. Ophthalmol. Vis. Sci. 2018;59(9):3518.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : The pathogenesis of primary angle closure glaucoma is poorly understood, although inflammation is considered to play a role in the evolution of the disease. The presence of peripheral anterior synechiae (PAS) in primary angle closure (PAC)/primary angle closure glaucoma (PACG) suggests that subclinical inflammation may be present in these eye. The aim of this study is to identify inflammation-associated proteins by analyzing the aqueous humour protein profile across primary angle closure disease spectrum.

Methods : Aqueous humour samples were obtained during elective phacoemulsification or combined phacoemulsification and trabeculectomy surgeries from 8 cataract (as controls) and 28 angle closure subjects (including 10 primary angle closure suspects (PACS), 9 PAC and 9 PACG). Quantitative proteomic analysis was performed using iTRAQ and liquid chromatography-mass spectrometry (LC-MS/MS).

Results : A total of 549 proteins were identified with false discovery rate (FDR) less than 1%.
Of the proteins identified in the aqueous humour, we found that the aqueous level of Complement C3 was significantly increased in the PAC (ratio 2.90±3.10, p=0.006) and PACG (ratio 3.11±2.60, p=0.005) groups compared to controls. Additionally, no significant differences were noted between the PACS group (ratio 1.38±1.18, p=0.19) and controls.

Conclusions : Our results demonstrate the existence of a distinctive inflammatory-related protein signature across the primary angle closure disease severity spectrum. This suggests that inflammation may play an important role in the development of PAC/PACG or progression from PACS, the earliest stage of the disease spectrum.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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