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Monisha Nongpiur, Lei Zhou, Siew Kwan Koh, Roger W. Beuerman, Eranga Vithana, Tin Aung, Tina Wong; Complement Factor 3 Profile in Aqueous Humour of Patients Across Primary Angle Closure Disease Spectrum. Invest. Ophthalmol. Vis. Sci. 2018;59(9):3518.
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© ARVO (1962-2015); The Authors (2016-present)
The pathogenesis of primary angle closure glaucoma is poorly understood, although inflammation is considered to play a role in the evolution of the disease. The presence of peripheral anterior synechiae (PAS) in primary angle closure (PAC)/primary angle closure glaucoma (PACG) suggests that subclinical inflammation may be present in these eye. The aim of this study is to identify inflammation-associated proteins by analyzing the aqueous humour protein profile across primary angle closure disease spectrum.
Aqueous humour samples were obtained during elective phacoemulsification or combined phacoemulsification and trabeculectomy surgeries from 8 cataract (as controls) and 28 angle closure subjects (including 10 primary angle closure suspects (PACS), 9 PAC and 9 PACG). Quantitative proteomic analysis was performed using iTRAQ and liquid chromatography-mass spectrometry (LC-MS/MS).
A total of 549 proteins were identified with false discovery rate (FDR) less than 1%.Of the proteins identified in the aqueous humour, we found that the aqueous level of Complement C3 was significantly increased in the PAC (ratio 2.90±3.10, p=0.006) and PACG (ratio 3.11±2.60, p=0.005) groups compared to controls. Additionally, no significant differences were noted between the PACS group (ratio 1.38±1.18, p=0.19) and controls.
Our results demonstrate the existence of a distinctive inflammatory-related protein signature across the primary angle closure disease severity spectrum. This suggests that inflammation may play an important role in the development of PAC/PACG or progression from PACS, the earliest stage of the disease spectrum.
This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.
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