July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
The anterior chamber of the eye as a clinical transplantation site of pancreatic islets for the treatment of diabetes: a study in a rat model of diabetes
Author Affiliations & Notes
  • Choun-Ki Joo
    Catholic Institutes of Visual Science, Catholic Univ Korea Coll of Med, Seoul, Korea (the Democratic People's Republic of)
    Seoul St. Mary's Hospital, Seoul, Korea (the Republic of)
  • Jae Young Lee
    Catholic Institutes of Visual Science, Catholic Univ Korea Coll of Med, Seoul, Korea (the Democratic People's Republic of)
    Seoul St. Mary's Hospital, Seoul, Korea (the Republic of)
  • Jeewon Mok
    Catholic Institutes of Visual Science, Catholic Univ Korea Coll of Med, Seoul, Korea (the Democratic People's Republic of)
  • ASIF IQBAL SHAWL
    Catholic Institutes of Visual Science, Catholic Univ Korea Coll of Med, Seoul, Korea (the Democratic People's Republic of)
  • Dong Won Paik
    Catholic Institutes of Visual Science, Catholic Univ Korea Coll of Med, Seoul, Korea (the Democratic People's Republic of)
  • Footnotes
    Commercial Relationships   Choun-Ki Joo, None; Jae Young Lee, None; Jeewon Mok, None; ASIF IQBAL SHAWL, None; Dong Won Paik, None
  • Footnotes
    Support  This research was supported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Education (2016R1A6A1A03010528)
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 3578. doi:
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      Choun-Ki Joo, Jae Young Lee, Jeewon Mok, ASIF IQBAL SHAWL, Dong Won Paik; The anterior chamber of the eye as a clinical transplantation site of pancreatic islets for the treatment of diabetes: a study in a rat model of diabetes. Invest. Ophthalmol. Vis. Sci. 2018;59(9):3578.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Diabetes is one of the most prevalent metabolic disorder usually detected by hyperglycemia due to insufficient insulin production from pancreatic β-cells or inadequate glucose uptake by peripheral cells, it affects almost 8-9% of the world population. Transplantation of insulin-producing cells has shown remarkable potential for treating patients with type1 diabetes. Since many clinical sites have been checked for the transplantation, we used the anterior chamber of eye due to its various advantages over other sites of transplantation to confirm diabetes control ability of islets

Methods : Pancreatic islets were transplanted into the anterior chamber of the diabetic rat(blood glucose≥400 mg/dl) with genetic background Sprague Dawley(SD) to compare islet dose(75-125 islet equivalents) and function in 6 rats. Diabetes was induced using single intraperitoneal streptozotocin(100 mg/kg) injection. Islets were prepared from healthy rats 1-2 days before transplantation and few islets were checked for various parameters like morphology, glucose induced Ca2+ rise and also glucose induced insulin secretion. Rats were checked for regular blood glucose levels and body weight before and after transplantation. Rats were also checked for IPGTT at 1month after transplantation

Results : In vitro, high glucose induced intracellular Ca2+ rise as well as insulin secretion in the beta cells. Our results show that islet transplantation into anterior chamber decreased blood glucose levels and increased weight gain compared to streptozotocin only model depending on islet dose transplanted. IPGTT showed ameliorated glucose in transplanted rats. Transplanted islets show vascularization by optical image. No major adverse effects on eye structure and functioning could be observed during the study

Conclusions : Our study demonstrates the short-term survival and function of pancreatic islets after transplantation into the anterior chamber of the diabetic rat eye. The safety and simplicity of this procedure and the ability to control diabetes provides support for future studies aimed at translating this procedure into the clinic for the potential of using the anterior chamber of the eye as a site for therapeutic transplantation in diabetes

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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