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Koji Ueda, Ryohei Nejima, Takashi Ono, Yukari Noguchi, Akiko Yagi, Takuya Iwasaki, Makoto Aihara, Kazunori Miyata; Profile and antibiotic susceptibility of bacterial isolates from infectious keratitis: 9-year experience in Japan. Invest. Ophthalmol. Vis. Sci. 2018;59(9):3679.
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© ARVO (1962-2015); The Authors (2016-present)
The widespread use of fluoroquinolones in the treatment of systemic and ocular diseases has led to significant levels of resistance. To clarify the trend of profile and antibiotic susceptibility of bacteria causing infectious keratitis, we performed a retrospective clinical study on the bacterial isolates from the corneal lesions of infectious keratitis in one hospital, Miyazaki, Japan.
Samples were obtained from corneal lesions from infectious keratitis from January 2008 to December 2016. Antibiotic susceptibility was measured using the disk diffusion method with KB disks (Eiken Chemical, Tokyo, Japan) from January 2008 to August 2013 and using the broth dilution method with SG17 plates (Eiken Chemical) from September 2013 to December 2016, and was determined in accordance with the methods of Clinical and Laboratory Standards Institute. Statistical analysis was performed using generalized estimating equation.
A total of 1211 consecutive corneal scrapings from 1166 patients (male, 40.6%, female, 59.4%; mean age, 50.4 years) were analyzed. 811 bacterial strains were isolated from 69.6% of the samples. Gram-positives bacteria accounted for 90.8% of bacterial isolates, including Propionibacterium acnes (45.8%) and Staphylococcus epidermidis (19.8%). The most common species of Gram-negative isolates was Serratia marcescens. Methicillin-resistant Staphylococcus aureus was 25.0% of the S. aureus isolates. 19.3% of the isolates were Levofloxacin (LVFX)-resistant isolates. S. epidermidis was the most common LVFX-resistant species (42.7%). Age-adjusted LVFX sensitivity rate among S. epidermidis showed a tendency of decrease from 71.5% of 2008 (n=23) to 37.4% of 2016 (n=14). (p=0.064)
We observed a tendency to increase in LVFX resistance in S. epidermidis isolates. Continued evaluation of fluoroquinolone sensitivity is essential to monitor for increasing resistance.
This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.
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