July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Investigation of the perioperative disturbances in the ocular surface microbiome using next-generation sequencing analysis
Author Affiliations & Notes
  • Hiroshi Eguchi
    Ophthalmology, Kindai University Sakai Hospital, Sakai, Osaka, Japan
  • Fumika Hotta
    Ophthalmology, Kindai University Sakai Hospital, Sakai, Osaka, Japan
  • Tomomi Kuwahara
    Molecular Microbiology, Kagawa University, Kida-gun, Kagawa, Japan
  • Haruyuki Imaohji
    Molecular Microbiology, Kagawa University, Kida-gun, Kagawa, Japan
  • Shunji Kusaka
    Ophthalmology, Kindai University Sakai Hospital, Sakai, Osaka, Japan
  • Yoshikazu Shimomura
    Ophthalmology, Kindai University, Osakasayama, Osaka, Japan
  • Footnotes
    Commercial Relationships   Hiroshi Eguchi, Alcon Japan (F), Novartis Pharma K. K. (F), Pfizer Japan Inc. (F); Fumika Hotta, None; Tomomi Kuwahara, None; Haruyuki Imaohji, None; Shunji Kusaka, Alcon Japan (F), Novartis Pharma K. K. (F), Pfizer Japan Inc. (F); Yoshikazu Shimomura, Daiichi Sankyo Co Ltd. (F), Pfizer Japan Inc. (F)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 3688. doi:
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    • Get Citation

      Hiroshi Eguchi, Fumika Hotta, Tomomi Kuwahara, Haruyuki Imaohji, Shunji Kusaka, Yoshikazu Shimomura; Investigation of the perioperative disturbances in the ocular surface microbiome using next-generation sequencing analysis
      . Invest. Ophthalmol. Vis. Sci. 2018;59(9):3688.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To investigate the disturbances in the ocular surface microbiome during the perioperative period following cataract surgery, using next-generation sequencing analysis.

Methods : Twenty-four eyes in 12 patients with no history of antibacterial eye drop use before the surgery were examined in this study. Ocular surface samples were collected before the surgery, 2 weeks after the surgery, and 4 weeks after the surgery. The perioperative use of antibiotic eye drops for prophylaxis against endophthalmitis was discontinued after sample collection, 2 weeks after the surgery. Bacterial 16S rDNA in the collected samples were amplified. Sequencing libraries were prepared by amplifying the V3-V4 region of the 16S rDNA. Sequencing was performed using the Illumina MiSeq platform (MiSeq Reagent Kit ver.3, 600 cycles) according to the manufacturer’s specifications. The diversity of micobiome at each time period was compared using the Shannon diversity coefficient.

Results : Proteabacteria, Firmicutes, and Bacteroides were the dominant before the surgery. At two weeks after the surgery, we found an increased number of Proteobacteria and fewer Firmicutes. This trend continued until 4 weeks after the surgery, even when antibiotic eye drop usage had been ceased. The Shannon coefficient at the preoperative stage, and 2 and 4 weeks after the surgery were 5.65, 5.12, and 4.95, respectively.

Conclusions : Disturbance in the ocular surface microbiome continue for 2 weeks after discontinuation of antibiotic eye drop use.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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