July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Effect of single IOP elevation on visual pathway
Author Affiliations & Notes
  • Viet Anh Nguyen Huu
    Ophthalmology, University of California, San Diego, La Jolla, California, United States
  • Lorena Raquel Rocha Jimenez
    Ophthalmology, University of California, San Diego, La Jolla, California, United States
  • Rebecca Dvorak
    Ophthalmology, University of California, San Diego, La Jolla, California, United States
  • Mary Jabari
    Ophthalmology, University of California, San Diego, La Jolla, California, United States
  • Robert N Weinreb
    Ophthalmology, University of California, San Diego, La Jolla, California, United States
  • Dorota Skowronska-Krawczyk
    Ophthalmology, University of California, San Diego, La Jolla, California, United States
  • Footnotes
    Commercial Relationships   Viet Anh Nguyen Huu, None; Lorena Raquel Rocha Jimenez, None; Rebecca Dvorak, None; Mary Jabari, None; Robert Weinreb, None; Dorota Skowronska-Krawczyk, None
  • Footnotes
    Support  NIH (R01 EY027011) and Richard C. Atkinson Lab for Regenerative Ophthalmology fund
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 3695. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Viet Anh Nguyen Huu, Lorena Raquel Rocha Jimenez, Rebecca Dvorak, Mary Jabari, Robert N Weinreb, Dorota Skowronska-Krawczyk; Effect of single IOP elevation on visual pathway. Invest. Ophthalmol. Vis. Sci. 2018;59(9):3695.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : To investigate short- and long-term effects of a single acute intraocular pressure (IOP) increase on the survival of retinal ganglion cells (RGCs) and on mouse vision as measured by visual evoked potential (VEP). Understanding these effects will potentially aid in tracking progression of glaucoma, in particular the influence of circadian IOP fluctuations on disease outcomes.

Methods : Unilateral intraocular hypertension was induced for 1 h to 30, 50 and 90 mmHg in wild-type mice, and their ocular health was analyzed after 7 and 21 days. RGC survival was assessed by fluorescent microscopy of Brn3a stained whole retina flat-mounts. Visual function loss was studied using VEP measurements.

Results : Acute intraocular pressure elevation resulted in RGC death correlative to IOP in all experimental groups at 7 days post-IOP. VEP showed functional losses following the same trend as RGC loss. Eyes showed minimal recovery in both health measures in the long-term cohort at day 21. Importantly, even a low IOP of 30 mmHg resulted in a significant, non-transient reduction in RGC count and the peak-to-peak amplitude of the N1 component of VEP.

Conclusions : These experiments demonstrated that the moderate IOP elevation recapitulated RGC loss and visual impairment observed in glaucoma patients and thus could be exploited as a tool to study glaucoma biology and to develop novel therapies.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×