July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Canonical Wnt signaling in optic nerve head
Author Affiliations & Notes
  • Elliott Allums
    University of North Texas Health Science Center, Fort Worth, Texas, United States
  • Yang Liu
    University of North Texas Health Science Center, Fort Worth, Texas, United States
  • Abbot F. Clark
    University of North Texas Health Science Center, Fort Worth, Texas, United States
  • Footnotes
    Commercial Relationships   Elliott Allums, None; Yang Liu, None; Abbot Clark, None
  • Footnotes
    Support  DOD grant (W81XWH-10-2-003) AND NIH training grant T32 AG020494
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 3739. doi:https://doi.org/
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    • Get Citation

      Elliott Allums, Yang Liu, Abbot F. Clark; Canonical Wnt signaling in optic nerve head. Invest. Ophthalmol. Vis. Sci. 2018;59(9):3739. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Canonical Wnt signaling pathway is an important signaling transduction mechanism involved in essential cellular processes. Optic nerve head (ONH) astrocytes play an important role in glaucoma pathogenesis. The role of Wnt pathway in ONH astrocytes has never been investigated. In this study, we examined the presence of canonical Wnt signaling pathway in ONH astrocytes.

Methods : Primary mouse optic nerve head (ONH) astrocytes from C57BL/6J mice were cultured and characterized using specific antibodies against glial (GFAP) and S100β. When confluent, cells were serum starved overnight and then treated for 24 hours with 100 nM Wnt3a. Following treatment, cells were collected and cytosolic and nuclear fractions were separated. Expression levels of β-catenin in fractions were determined by western blotting. Images were analyzed via densitometry and fold changes in expression were compared to control group.

Results : Cells cultured from mouse ONH were positive for GFAP and S100β. Expression levels of β-catenin increased 1.354-fold when treated with 100 nM Wnt3a compared to control in the cytosolic fraction and 1.145-fold when treated with 100 nM Wnt3a compared to control in the nuclear fraction.

Conclusions : Wnt3a promoted nuclear translocation of β-catenin in mouse ONH astrocytes, which suggests a functional canonical Wnt signaling pathway in mouse ONH. Functions of this pathway in ONH astrocytes will be further studied.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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