July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Reduced intraepithelial corneal nerve density and sensitivity accompany acute and chronic dry eye in C57BL/6 mice
Author Affiliations & Notes
  • Mary Ann Stepp
    Anatomy & Regenerative Biology, George Washington University, Washington, District of Columbia, United States
  • Sonali Pal-Ghosh
    Anatomy & Regenerative Biology, George Washington University, Washington, District of Columbia, United States
  • Gauri Tadvalkar
    Anatomy & Regenerative Biology, George Washington University, Washington, District of Columbia, United States
  • Alexa R. Williams
    Anatomy & Regenerative Biology, George Washington University, Washington, District of Columbia, United States
  • Stephen C Pflugfelder
    Dept of Ophthalmology, Baylor College of Medicine, Houston, Texas, United States
  • Cintia S De Paiva
    Dept of Ophthalmology, Baylor College of Medicine, Houston, Texas, United States
  • Footnotes
    Commercial Relationships   Mary Ann Stepp, None; Sonali Pal-Ghosh, None; Gauri Tadvalkar, None; Alexa R. Williams, None; Stephen Pflugfelder, Senju (C), Shire (C); Cintia De Paiva, None
  • Footnotes
    Support  This work was supported by NIH/NEI EY011915 (SCP), NIH Training Grant T32-AI053831 (FB), RPB Research to Prevent Blindness, The Oshman Foundation, William Stamps Farish Fund and The Hamill Foundation. In addition, the work was also supported by NIH/NEI EY08512 and EY021784.  
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 3875. doi:
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      Mary Ann Stepp, Sonali Pal-Ghosh, Gauri Tadvalkar, Alexa R. Williams, Stephen C Pflugfelder, Cintia S De Paiva; Reduced intraepithelial corneal nerve density and sensitivity accompany acute and chronic dry eye in C57BL/6 mice. Invest. Ophthalmol. Vis. Sci. 2018;59(9):3875.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To determine the impact of acute and chronic murine dry eye disease on intraepithelial corneal nerve density and function

Methods : Acute desiccating stress was induced using scopolamine, low humidity and airflow. Mice were sacrificed at 3, 5, and 10 days; chronic dry eye was induced by aging (24 months). Axon density, thickness, apical axon terminal extension, and corneal touch sensation were determined. In addition, RNA was isolated from corneal epithelial cells of young and 24-month-old mice and QPCR performed to quantify mRNAs for genes involved in axon growth and targeting.

Results : Both acute and chronic dry eye reduce axon density and thickness as well as corneal touch sensation. Acute dry eye reduces axon terminal extension but chronic dry eye does not. In 24 month old mice, mRNAs for several genes that target axon growth and one that mediates axon retraction are altered in expression but that that mediate axon turnover are not altered.

Conclusions : Acute and chronic dry eye disease impact sensory axon density and function. QPCR data implicate altered corneal epithelial cell expression of proteins that mediate axon elongation and/or retraction.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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