July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Hybrid Retina: A Novel Concept for Sight Restoration
Author Affiliations & Notes
  • Yossi Mandel
    School of Optometry and Vision Science, Bar-Ilan University, Ramat Gan, Israel, Israel
    Bar-Ilan Institute for Nanotechnology and Advanced material - BINA, Bar-Ilan University, Ramat Gan, Israel, Israel
  • Amos Markus
    School of Optometry and Vision Science, Bar-Ilan University, Ramat Gan, Israel, Israel
  • Gal Shpun
    School of Optometry and Vision Science, Bar-Ilan University, Ramat Gan, Israel, Israel
    Bar-Ilan Institute for Nanotechnology and Advanced material - BINA, Bar-Ilan University, Ramat Gan, Israel, Israel
  • Revital Schick
    School of Optometry and Vision Science, Bar-Ilan University, Ramat Gan, Israel, Israel
  • Yoav Chemla
    School of Optometry and Vision Science, Bar-Ilan University, Ramat Gan, Israel, Israel
    Bar-Ilan Institute for Nanotechnology and Advanced material - BINA, Bar-Ilan University, Ramat Gan, Israel, Israel
  • Itai Henn
    School of Optometry and Vision Science, Bar-Ilan University, Ramat Gan, Israel, Israel
    Bar-Ilan Institute for Nanotechnology and Advanced material - BINA, Bar-Ilan University, Ramat Gan, Israel, Israel
  • Nairouz Farah
    School of Optometry and Vision Science, Bar-Ilan University, Ramat Gan, Israel, Israel
  • Footnotes
    Commercial Relationships   Yossi Mandel, None; Amos Markus, None; Gal Shpun, None; Revital Schick, None; Yoav Chemla, None; Itai Henn, None; Nairouz Farah, None
  • Footnotes
    Support  Israeli Ministry of Defence
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 3974. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Yossi Mandel, Amos Markus, Gal Shpun, Revital Schick, Yoav Chemla, Itai Henn, Nairouz Farah; Hybrid Retina: A Novel Concept for Sight Restoration. Invest. Ophthalmol. Vis. Sci. 2018;59(9):3974.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : To overcome the large neuron-electrode distance and the non-selective activation of various retinal pathways (e.g. ON and OFF) which limit the efficiency of currently available retinal prostheses, we propose a novel approach based on coupling glutamatergic neuron to high-density electrode arrays, which we term Hybrid Retinal Prosthesis. The coupled cells will synapse with the host retina and electrical stimulation of the implant induce the activation of retinal circuitry at a cellular resolution with visual restoration at high acuity.

Methods : We generated photoreceptor precursors from human embryonic stem cells, to serve as glutamatergic neurons, by a combination of IWR1e, SAG, CHIR99021 in agarose microwells. COMSOL Multyphysics computer modeling was utilized to evaluate and optimize the effect of various geometrical parameters on neurons activation charge. To electro-physiologically characterize the generated cells we employed calcium imaging (rhod2) to visualize intracellular calcium dynamics in response to electrical activation. Finally, optimization and characterization of the neuron-electrode interface were performed by advanced imaging techniques, on electrode arrays which were fabricated by our group.

Results : Cell differentiation efficiency was over 80%, as shown by Fluorescence-activated cell sorting to the photoreceptor marker CRX. Simulations revealed that charge thresholds could be reduced to 0.03nC in a configuration where neurons are sealed in a microwell, as compared to 49nC in a flat electrode configuration. Maturation of neurons was demonstrated by the 3-150 fold increase in the expression of sodium, potassium and calcium voltage-sensitive channels at 12w of differentiation in addition to evidence of voltage-dependent currents observed in voltage clamp recordings. A marked increase and decrease in intracellular calcium was observed following cathodic and anodic electrical pulses, respectively. Finally, surface treatment of electrodes with RGD resulted in a two-fold increase in electrode-neural cells growth area and decreased the electrode-neuron cleft, as revealed by scanning electrode and focused ion beam microscopy.

Conclusions : We propose a new strategy for vision restoration with a hybrid retinal implant composed of a high-density electrode array incorporated with glutamatergic neurons. The hybrid device has the potential to mimic many features of natural vision, and restore vision at high resolution and quality .

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×