July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
The antioxidative effects of long pentraxin 3 involve an increase in antioxidative enzymes, catalase and glucose-6-phosphate dehydrogenase, in human retinal pigment epithelial cells
Author Affiliations & Notes
  • Je Moon Woo
    Ulsan university hospital, Ulsan, Korea (the Republic of)
    University of Ulsan College of Medicine, Ulsan, Korea (the Republic of)
  • NARAE HWANG
    College of Natural Sciences, University of Ulsan , Ulsan, Korea (the Republic of)
  • Su Wol Chung
    College of Natural Sciences, University of Ulsan , Ulsan, Korea (the Republic of)
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 3987. doi:
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      Je Moon Woo, NARAE HWANG, Su Wol Chung; The antioxidative effects of long pentraxin 3 involve an increase in antioxidative enzymes, catalase and glucose-6-phosphate dehydrogenase, in human retinal pigment epithelial cells. Invest. Ophthalmol. Vis. Sci. 2018;59(9):3987.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To investigate the role of long pentraxin 3(PTX3) during oxidative stress, the expression levels of PTX3 were analyzed in the presence of sodium iodate(NaIO3), oxidative stress inducer in human retinal pigment epitheal (RPE) cells.

Methods : Primary human RPE and ARPE-19 cells were treated with NaIO3 in time-(12, 24, 48 hours) and dose-dependent (0.05, 0.1, 0.5, 2.5 mM) manners. mRNA and protein levels of PTX3 were measured using quantitative real-time reverse transcription-polymerase chain rection(qRT-PCR) and enzyme-linked immunosorbent assay(ELISA) after NaIO3 treatment. The specific inhibitors were used to determine signaling pathways of oxidative stress-induced PTX3 expression. The expression level of antioxidant enzymes (catalase, glucose-6-phosphate dehydrogenease, superoxide dismutase, glutathione peroxidase) was analyzed using qRT-PCR after NaIO3 treatment in PTX3 shRNA or control shRNA in ARPE-19 cells.

Results : In this study, mRNA and protein expression levels of PTX3 were incresed with NaIO3 treatment for the time-and dose-dependently in both primary human RPE and ARPE-19 cells. Also oxidative stress-induced PTX3 protein was suppressed in the presence of phosphoinositide 3-kinase inhibitor (LY2940002) and reactiveoxygen species (ROS) scavenger (N-acetyl-L-cysteine, NAC) in primary human RPE cells. The mRNA expression of catalase and glucose-6-phosphate dehydrogenase was incresed after NaIo3 treatment in primary human RPE cells. However, the mRNA expression of catalase and glucose-6-phosphate dehydrogenase was decresed in PTX3 shRNA ARPE-19 cells compared with control shRNA ARPE-19 cells.

Conclusions : The expression levels of PTX3 were increased during oxidative stress in human RPE cells. Also, the expression of oxidative stress-induced antioxidant enzymes was decreased in PTX3 shRNA ARPE-19 cells. These data suggested that PTX3 induces during oxidative stress and may have antioxidatve effects.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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