July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Correlation between fibronectin, connective tissue growth factor and periostin in preretinal fibrovascular proliferation
Author Affiliations & Notes
  • Yuki Kubo
    Ophthalmology, Kyushu University, Fukuoka, Fukuoka, Japan
  • Shigeo Yoshida
    Ophthalmology, Kyushu University, Fukuoka, Fukuoka, Japan
  • Yuya Fujii
    Ophthalmology, Kyushu University, Fukuoka, Fukuoka, Japan
  • Yoshiyuki Kobayashi
    Ophthalmology, Kyushu University, Fukuoka, Fukuoka, Japan
  • Takahito Nakama
    Ophthalmology, Kyushu University, Fukuoka, Fukuoka, Japan
  • Keijiro Ishikawa
    Ophthalmology, Kyushu University, Fukuoka, Fukuoka, Japan
  • Shintaro Nakao
    Ophthalmology, Kyushu University, Fukuoka, Fukuoka, Japan
  • Toshio Hisatomi
    Ophthalmology, Kyushu University, Fukuoka, Fukuoka, Japan
  • Yasuhiro Ikeda
    Ophthalmology, Kyushu University, Fukuoka, Fukuoka, Japan
  • Tatsuro Ishibashi
    Ophthalmology, Kyushu University, Fukuoka, Fukuoka, Japan
  • Koh-hei Sonoda
    Ophthalmology, Kyushu University, Fukuoka, Fukuoka, Japan
  • Footnotes
    Commercial Relationships   Yuki Kubo, None; Shigeo Yoshida, None; Yuya Fujii, None; Yoshiyuki Kobayashi, None; Takahito Nakama, None; Keijiro Ishikawa, None; Shintaro Nakao, None; Toshio Hisatomi, None; Yasuhiro Ikeda, None; Tatsuro Ishibashi, None; Koh-hei Sonoda, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 4290. doi:
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      Yuki Kubo, Shigeo Yoshida, Yuya Fujii, Yoshiyuki Kobayashi, Takahito Nakama, Keijiro Ishikawa, Shintaro Nakao, Toshio Hisatomi, Yasuhiro Ikeda, Tatsuro Ishibashi, Koh-hei Sonoda; Correlation between fibronectin, connective tissue growth factor and periostin in preretinal fibrovascular proliferation. Invest. Ophthalmol. Vis. Sci. 2018;59(9):4290.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Our previous study revealed that periostin (PN) plays a major role in preretinal fibrovascular proliferation. We also showed that the gene expression level of fibronectin (FN) and connective tissue growth factor (CTGF) was elevated in preretinal membranes from eyes of proliferative vitreoretinopathy patients by genome-wide gene expression profiling. The purpose of this study was to determine the correlation among FN, CTGF and PN in preretinal fibrovascular proliferation.

Methods : PN was overexpressed in human retinal pigment epithelial cells (hRPEs), and PN-protein complex was extracted. We performed mass spectrometry to determine the protein components binding to PN. Real time RT-PCR was performed to determine the level of mRNA of PN, FN and CTGF from hRPEs and fibrovascular membranes (FVMs) obtained from patients with proliferative diabetic retinopathy (PDR) by vitrectomy. We also measured expression levels of these proteins in vitreous of PDR patients by enzyme-linked immunosorbent assay (ELISA). Immunostaining of FVMs from PDR patients and from the mouse model of oxygen-induced retinal neovascularization (OIR) was performed.

Results : By mass spectrometry, the expression level of FN in the protein complex was elevated (score 1950, match 74). The expression levels of PN, FN and CTGF mRNA in the FVMs were significantly higher than those in control samples (p<0.05). The mRNA levels of PN, FN and CTGF increased and peaked at twelve, twelve and three hours, respectively, after TGF-β stimulation. The protein levels of vitreous humor were significantly correlated between FN, CTGF and PN (p<0.001, ρ=0.75, p<0.01, ρ=0.48, respectively). FN, CTGF and PN was co-stained in the alpha-SMA positive cells in FVMs.

Conclusions : These results indicate that CTGF and FN interact with PN and regulate the formation of preretinal FVMs.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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