July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Effect of photobiomodulation on wound healing of corneal epithelium through Rho-GTPase
Author Affiliations & Notes
  • Kyong Jin Cho
    Ophthalmology, College of Medicine, Dankook University, Cheonan, Chungnam, Korea (the Republic of)
  • Chang Rae Rho
    Ophthalmology and Visual Science, Daejeon St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea (the Republic of)
  • Bong Hui Kang
    Neurology, College of Medicine, Dankook University, Cheonan, Korea (the Republic of)
  • Footnotes
    Commercial Relationships   Kyong Jin Cho, None; Chang Rae Rho, None; Bong Hui Kang, None
  • Footnotes
    Support  Supported by grants by the National Research Foundation of Korea (NRF) grant funded by the Korean government (NRF-2016R1C1B2016649)
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 4365. doi:
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      Kyong Jin Cho, Chang Rae Rho, Bong Hui Kang; Effect of photobiomodulation on wound healing of corneal epithelium through Rho-GTPase. Invest. Ophthalmol. Vis. Sci. 2018;59(9):4365.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : For recent years, photpbiomodulation (PBM) using low level light has been applied to diverse clinical approach since PBM had a potent to elevate cell metabolism or to regulate various signaling pathway. The aim of this study was to investigate the possibility of PBM effect on the wound healing of corneal epithelial cells in a short time.

Methods : To investigate which wavelength was most effective to corneal epithelium wound healing, the wavelength at 470, 530, 660, 740, and 850 nm of light emitting diode (LED) arrays were used. We assessed the proliferative effect by MTT assay, cell cycle assay, BrdU immunofluorescence (IF) staining and the motility effect by wound healing assay after PBM. We also evaluated the cytoskeletal
rearrangement effect of PBM by Westernblot analysis and IF staining.

Results : PBM had no effect to cell proliferation; cell cycle portion and BrdU were not changed after PBM treatment whereas cell survival was decreased at 470 nm. However, PBM had an effect to migration over 660 nm of wavelength, specially 740 nm was most effective. The expression of Rho A and Rho C was increased after PBM over 660 nm. The expression of cdc42 and mTORC2 showed no differences.

Conclusions : Our study revealed that PBM could increase corneal epithelial cell migration capacity without cell proliferation in short time via activation of a part of Rho-GTPase pathways without effect of upstream signals. These findings may be used for future development of PBM-based therapy for acute ocular surface diseases.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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