July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Keratocyte-derived canonical Wnt signaling modulates corneal wound healing
Author Affiliations & Notes
  • Yen-Chiao Wang
    School of Optometry, Indiana University, Bloomington, Indiana, United States
  • Yujin Zhang
    School of Optometry, Indiana University, Bloomington, Indiana, United States
  • Lingling Zhang
    School of Optometry, Indiana University, Bloomington, Indiana, United States
  • Yuka Okada
    School of Medicine, Wakayama Medical University, Wakayama, Japan
  • Chia-Yang Liu
    School of Optometry, Indiana University, Bloomington, Indiana, United States
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 4366. doi:
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    • Get Citation

      Yen-Chiao Wang, Yujin Zhang, Lingling Zhang, Yuka Okada, Chia-Yang Liu; Keratocyte-derived canonical Wnt signaling modulates corneal wound healing. Invest. Ophthalmol. Vis. Sci. 2018;59(9):4366.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Corneal stromal-epithelial interaction plays pivotal role in the maintenance of corneal homeostasis. This study is to determine whether keratocyte-derived Wnt singling plays a role in corneal epithelial debridement wound healing.

Methods : A Doxycycline (Dox)-inducible quadruple transgenic mouse line (KeraRT; TetO-Cre; Ctnnb1fE3; Axin2LacZ) was generated to conditionally expressing a b-catenin gain-of-function mutant (Ctnnb1ΔE3) and to simultaneously monitoring Wnt signaling activity by the knock-in allele Axin2LacZ. A 2 mm debridement wound was made in the corneal epithelium of the adult experimental mice. Wound healing was monitored between 0 h and 48 h post-debridement via fluorescein uptake. Collected eyeballs were subjected to examination by X-Gal, H&E, IHC staining and western blots.

Results : X-gal staining showed that endogenous canonical Wnt signaling activity is dramatically decreased during the whole process of corneal epithelial debridement wound healing. Expression of Ctnnb1ΔE3 in stromal keratocytes retarded corneal wound healing via inhibition of epithelial cell proliferation. IHC staining suggested that TGFb signaling was inhibited by ectopic canonical Wnt signaling activation in the corneal stroma during wound healing. Phalloidin staining did not reveal any striking distinctions in cell shape between the mutant and wildtype mice.

Conclusions : These data implicate keratocyte-derived canonical Wnt signaling plays a role in corneal epithelial wound healing.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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