July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Label-free absolute quantification proteomic analysis of vitreous humor from patients with high myopia
Author Affiliations & Notes
  • Fenghua Wang
    Ophthalmology, Shanghai General Hospital , Shanghai Jiao Tong University School of Medicine, Shanghai, China
    Shanghai Engineering Center for Visual Science and Photomedicine, Shanghai, China
  • Minlu Song
    Ophthalmology, Shanghai General Hospital , Shanghai Jiao Tong University School of Medicine, Shanghai, China
  • Yanping Zhou
    Ophthalmology, Zhongshan Hospital, Fudan University, Shanghai, China
  • Meichun Xiao
    Ophthalmology, Shanghai General Hospital , Shanghai Jiao Tong University School of Medicine, Shanghai, China
  • Xiaodong Sun
    Ophthalmology, Shanghai General Hospital , Shanghai Jiao Tong University School of Medicine, Shanghai, China
    Shanghai Engineering Center for Visual Science and Photomedicine, Shanghai, China
  • Footnotes
    Commercial Relationships   Fenghua Wang, None; Minlu Song, None; Yanping Zhou, None; Meichun Xiao, None; Xiaodong Sun, None
  • Footnotes
    Support  NSFC Grant 81470640
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 4738. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Fenghua Wang, Minlu Song, Yanping Zhou, Meichun Xiao, Xiaodong Sun; Label-free absolute quantification proteomic analysis of vitreous humor from patients with high myopia. Invest. Ophthalmol. Vis. Sci. 2018;59(9):4738.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : High myopia significantly increases the possibility of developing a variety of retinal complications, leading to severe vision loss. To offer clues of pathogenic mechanisms related to high myopia, we compared the vitreous humor proteome between patients with high myopia and idiopathic macular hole (MH).

Methods : Vitreous humor samples were obtained from patients with highly myopic MH (n=5) and idiopathic MH (n=5) during pars plana vitrectomy. Samples were digested and mixed with internal standards, Universal Proteomics Dynamic Range Standard (UPS2) peptides. Mixtures were then analyzed by nano-liquid chromatography-tandem mass spectrometry (nano-LC-MS/MS). Label-free quantification and intensity-based absolute quantification (iBAQ) were employed for protein quantification. We identified differential expressed proteins (abundance ratio > 1.5) by bioinformatic analysis. Western blot was performed to validate the differential expressions of representative proteins.

Results : An average of 461 proteins were identified and quantified per sample. Comparative analysis revealed 83 differential expressed proteins. Among these, the abundance of 48 and 35 proteins were significantly higher and lower in high myopia compared with controls. Extracellular region was the component in which most of the specific subset of proteins located. Biological pathway analysis suggested that most of the differentially expressed proteins were related to complement and coagulation cascades, inflammatory responses, extracellular matrix-receptor interaction and focal adhesion. The differential expressions of opticin, apolipoprotein A1 and complement C1q were confirmed by Western blot.

Conclusions : This study reveals the differences in the composition of vitreous proteins between high myopia and non-myopia. Changes of biological pathways, including complement activation, may involve in the pathogenesis of high myopia. The identification of the key proteins could be important for the development of prognosis and intervention.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×