July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Association between meibomian lipid profiles and age-related changes in gland morphology and function
Author Affiliations & Notes
  • Nisha Shirish Yeotikar
    Clinical & Research Trials Centre, Brien Holden Vision Institute, Kensington, New South Wales, Australia
  • Judith Flanagan
    Research & Development, Brien Holden Vision Institute, Sydney, New South Wales, Australia
    School of Optometry & Vision Science, University of New South Wales, Sydney, New South Wales, Australia
  • Thomas John Naduvilath
    Clinical & Research Trials Centre, Brien Holden Vision Institute, Kensington, New South Wales, Australia
  • Maria Markoulli
    School of Optometry & Vision Science, University of New South Wales, Sydney, New South Wales, Australia
  • Daniel Tilia
    Clinical & Research Trials Centre, Brien Holden Vision Institute, Kensington, New South Wales, Australia
    School of Optometry & Vision Science, University of New South Wales, Sydney, New South Wales, Australia
  • Eric B Papas
    School of Optometry & Vision Science, University of New South Wales, Sydney, New South Wales, Australia
  • Footnotes
    Commercial Relationships   Nisha Yeotikar, None; Judith Flanagan, None; Thomas Naduvilath, None; Maria Markoulli, None; Daniel Tilia, None; Eric Papas, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 4875. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Nisha Shirish Yeotikar, Judith Flanagan, Thomas John Naduvilath, Maria Markoulli, Daniel Tilia, Eric B Papas; Association between meibomian lipid profiles and age-related changes in gland morphology and function. Invest. Ophthalmol. Vis. Sci. 2018;59(9):4875.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : The aim of the study was to investigate changes in the profile of meibum lipids and concurrent alterations in meibomian gland (MG) function and morphology as a function of age and diet, in a normal adult population.

Methods : 185 subjects (109 females) within the age range of 25-66 years were examined specifically for MG function (meibum quality and gland expressibility), morphology (gland dropout) and dry eye disease signs, during a single clinical visit. Meibum samples were collected from both eyes, pooled and analysed using nano-electrospray ionization tandem mass spectrometry. A food frequency questionnaire (FFQ, Cancer Council Victoria, Australia), covering 74 food items, was administered to assess habitual eating habits over one year. Lipid data were normalized and log transformed prior to data analysis.

Results : Meibum samples revealed the following lipid classes for the group as a whole: cholesterol esters (CEs, 60.7%), wax esters (WEs, 33.7%), (O-acyl)-omega-hydroxy fatty acids (OAHFAs, 3.3%), tri-acyl glycerides (TAGs, 2.2%) and ceramides (0.1%). Apart from CEs (nine subclasses), whose mean concentration reduced as age increased (p < 0.01), no other lipid class showed any statistically significant association with age or sex. A statistically significant reduction in the mean concentration of ten subclasses of OAHFAs, as well as TAGs and WE subtypes, was found with reduction in MG expressibility (p < 0.03). Five subclasses of OAHFAs also showed significant reduction in the mean concentration with increase in MG dropout (p < 0.02). Meibum quality and tear function variables did not show any association with MG lipids. From the FFQ, the distribution of the main food groups (log transformed) in the cohort diet (per day) was: carbohydrates (5.07 g), all fats (4.12 g), proteins (4.3 g), and fibre (2.96 g). There was a weak positive correlation between fiber and vitamin C intake with all meibum lipids classes (r<0.217, p=0.003 and r<0.209, p=0.005, respectively). None of the fatty acids was associated with any meibum lipid class (p>0.052).

Conclusions : Cholesterol esters in meibum lipids tend to decrease with aging. OAHFAs in meibum lipids tend to be lower with decreased MG expressibility and increased gland dropout. The FFQ does not show any association between MG lipids and diet in this normal cohort without any ocular pathology.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×