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Zhengri Li, wei Li, Zuguo Liu, rongrong zong, Lian Cui, Ying Li, Kyung Chul Yoon; Expression of NOD-like receptor and clinical correlations in patients with dry eye disease.. Invest. Ophthalmol. Vis. Sci. 2018;59(9):4893.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the expression pattern of Nucleotide binding and oligomerization domain (NOD)-like receptor which detects danger intracellular signaling and its correlation with dry eye markers.
We recruited 23 participants with SS dry eye, 14 participants with non-SS dry eye, and 13 healthy controls without dry eye symptoms. Ocular surface disease index (OSDI) was self-answered and clinical tests including tear-film breakup time (BUT), Schirmer I test, cornea fluorescein staining (CFS) were performed. Specimens were obtained from the upper tarsal conjunctiva of each participant using a modified impression cytology method. NOD-1, Nuclear Factor Kappa B, and Ikka expression was determined by real-time RT-PCR and western blot. The correlations between NOD-1 and ocular surface parameters were analyzed.
Patients in the SSDE group had significantly higher OSDI, TBUT, Schirmer’s test, CFS compared with the NSSDE group. The level of NOD-1, NF-kB and Ikka had significant differences between the SSDE group and NSSDE group.The expression of the NOD-1 was significantly correlated with OSDI, Schirmer’s test, and CSS, whereas it was not significantly correlated with TUBT.
Expression of NOD-1 might play a key role for initiating the inflammatory response in dry eye and its correlation with dry eye markers.
This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.
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