July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Repeatability of vessel density measurements of high-density optical coherence tomography angiography scans and their comparability with standard-density scans in normal and glaucoma eyes
Author Affiliations & Notes
  • Harsha L Rao
    Glaucoma, Narayana Nethralaya, Bangalore, India
  • Jayasree P Venugopal
    Glaucoma, Narayana Nethralaya, Bangalore, India
  • Robert N Weinreb
    Ophthalmology, University of California San Diego, San Diego, California, United States
  • Zia Pradhan
    Glaucoma, Narayana Nethralaya, Bangalore, India
  • Narendra K Puttaiah
    Glaucoma, Narayana Nethralaya, Bangalore, India
  • Sathi Devi
    Glaucoma, Narayana Nethralaya, Bangalore, India
  • Kaweh Mansouri
    Glaucoma Center, Montchoisi Clinic, Swiss Vision Network, Lausanne, Switzerland
  • Carroll A B Webers
    University Eye Clinic Maastricht, University Medical Center, Maastricht, Netherlands
  • Footnotes
    Commercial Relationships   Harsha Rao, None; Jayasree Venugopal, None; Robert Weinreb, Optovue (S); Zia Pradhan, None; Narendra Puttaiah, None; Sathi Devi, None; Kaweh Mansouri, None; Carroll Webers, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 5068. doi:
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      Harsha L Rao, Jayasree P Venugopal, Robert N Weinreb, Zia Pradhan, Narendra K Puttaiah, Sathi Devi, Kaweh Mansouri, Carroll A B Webers; Repeatability of vessel density measurements of high-density optical coherence tomography angiography scans and their comparability with standard-density scans in normal and glaucoma eyes. Invest. Ophthalmol. Vis. Sci. 2018;59(9):5068.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To evaluate the repeatability of peripapillary vessel density (VD) measurements of high-density (HD) optical coherence tomography angiography (OCTA, RTVue XR OCT, Optovue Inc.) scans in normal and glaucomatous eyes, and to compare these VD measurements with those of standard-density (SD) OCTA scans.

Methods : In a cross-sectional study, 46 normal (33 subjects) and 89 glaucoma eyes (64 patients) underwent 3 HD (400x400 A-lines) and 1 SD (304x304 A-lines) optic nerve head OCTA scans (4.5x4.5 mm) in the same session. Repeatability of the VD measurements of HD scans was assessed using within-subject coefficient of repeatability (CRw) and coefficient of variation (CVw). Effect of signal strength index (SSI) on repeatability was evaluated with repeated measures mixed effects models. Agreement in VD measurements between HD and SD scans was assessed using Bland and Altman analysis.

Results : CRw (%) and CVw (%) of peripapillary VD measurements of HD scans ranged from 3.2 to 6.7, and 2.6 to 4.8 respectively in glaucoma eyes. Repeatability estimates of most VD measurements were better in normal compared to glaucoma eyes. VD measurements significantly increased with increase in SSI of repeat scans (coefficients ranging from 0.17 to 0.37, p<0.001 for all associations). Therefore, the HD scan with a SSI closest to that of the SD scan, was used in the agreement analysis. Mean difference in the VD values between HD and SD scans ranged between 0.7% (temporal sector VD in normal) and 2.4% (superonasal sector VD in glaucoma), with HD scans showing significantly higher VD values than SD scans. The 95% limits of agreement (LoA) in glaucoma eyes ranged between -2.0% to 5.0% for whole enface VD and -4.8% to 9.6% for superotemporal VD.

Conclusions : Test-retest repeatability of VDs on HD scans was as high as 6%. VD of HD scans were higher than that of SD scans. The wide 95% LoA indicate that the VD measurements of HD and SD scans cannot be used interchangeably. These results should be considered while using OCTA for longitudinal evaluation in glaucoma.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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