Investigative Ophthalmology & Visual Science Cover Image for Volume 59, Issue 9
July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
The intrinsically-photosensitive retinal ganglion cell (ipRGC)-mediated pupil light reflex (PLR) as a potential biomarker for sleep and cognition
Author Affiliations & Notes
  • Yanjun Chen
    Ophthalmology and Visual Sciences, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin, United States
  • Alex Pinto
    Ophthalmology and Visual Sciences, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin, United States
  • Adam J Paulsen
    Ophthalmology and Visual Sciences, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin, United States
  • Karen J Cruickshanks
    Ophthalmology and Visual Sciences, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin, United States
  • Footnotes
    Commercial Relationships   Yanjun Chen, None; Alex Pinto, None; Adam Paulsen, None; Karen Cruickshanks, None
  • Footnotes
    Support  The study is supported by NIA, R01AG021917, unrestricted grant from Research to Present Blindness, University of Wisconsin Department of Ophthalmology and Visual Sciences research funding, and F.A. Davis Fund for vision research
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 5164. doi:
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    • Get Citation

      Yanjun Chen, Alex Pinto, Adam J Paulsen, Karen J Cruickshanks; The intrinsically-photosensitive retinal ganglion cell (ipRGC)-mediated pupil light reflex (PLR) as a potential biomarker for sleep and cognition. Invest. Ophthalmol. Vis. Sci. 2018;59(9):5164.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Recent discovery of the ipRGC, a small group of melanopsin-containing retinal photoreceptors, has supported the critical role of the eye in the regulation of non-visual physiologic functions such as sleep and pupil reactivity. Here, we present a preliminary analysis to characterize the ipRGC-mediated PLR in an epidemiologic cohort.

Methods : We are conducting a cross-sectional study to collect pupil reactivity in a subset of participants in the 10-year follow up examination of the Beaver Dam Offspring Study (BOSS), an established, large longitudinal epidemiologic, multi-sensory study of aging. The pupil reactivity is recorded using a binocular infrared pupillometer (DP2000 Human Laboratory Pupillometer, Neur-Optics, Inc., Irvine, CA). The stimulus consists of a pair of 1-sec red (640±10 nm) and blue (467±17 nm) light stimulus at the intensity of 2.0 log lux. The ipRGC-mediated PLR is calculated as post-illumination pupil response (PIPR) at 6-sec after termination of the 1-sec light stimulus. Sleep quality is assessed using a modified, semi-quantified Wisconsin Sleep Cohort Study questionnaire. Cognitive function testing consists of Mini-mental State Examination (MMSE), Trail Making Test A and B, Rey’s Auditory Verbal Learning Test (AVLT), Digit Symbol Substitution Test, and Verbal Fluency Test (F, A and S).

Results : 208 BOSS participants were included in this preliminary analysis, with age ranging from 37 to 80 years (mean±SD: 59.4±9.5). The baseline pupil diameter decreases with increasing age (linear regression, y=-0.041x+7.777, R2=0.19). The percent PIPR demonstrates a modest age-related decline, and a stronger association with baseline pupil diameter (linear regression, y=0.038x+0.042, R2=0.20).

Conclusions : The preliminary analysis of this selected BOSS cohort demonstrated a modest trend of age -related decline in percent PIPR and a positive correlation between the percent PIPR with baseline pupil diameter. The correlation of the ipRGC-mediated PLR with sleep and cognitive measurement will be explored further upon completion of data collection in Dec 2017.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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