July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
In vivo and ex vivo multi-modal imaging of ocular inflammation in animal models of disease
Author Affiliations & Notes
  • Samantha Dando
    Monash Biomedicine Discovery Institute and Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia
  • Footnotes
    Commercial Relationships   Samantha Dando, None
  • Footnotes
    Support  NHMRC Preterm Infants CRE PG 0930/17702004
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 5339. doi:
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      Samantha Dando; In vivo and ex vivo multi-modal imaging of ocular inflammation in animal models of disease. Invest. Ophthalmol. Vis. Sci. 2018;59(9):5339.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Presentation Description : The transparent nature of the mouse eye offers unique advantages for in vivo visualisation and ex vivo imaging of ocular tissue. Combined with the use of fluorescent reporter mice, these techniques enable exquisite imaging of ocular inflammation and immune cells in various disease models. The use multi-modal fundus imaging in particular has significantly improved our understanding of the pathogenesis of mouse models of uveitis, including experimental autoimmune uveitis (EAU) and endotoxin-induced uveitis (EIU). Using a range of reporter mice (Cx3cr1-gfp/+, LysM-eGFP, CD11c-eYFP) and a combination of in vivo and ex vivo imaging, our laboratory has characterised the dynamic nature of inflammatory changes during EAU and EIU. This presentation will discuss these findings and highlight the potential role of specific immune cell subsets in driving disease pathogenesis.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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