July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018

Temporal properties of flicker ERGs in a transgenic rabbit model of retinitis pigmentosa
Author Affiliations & Notes
  • Shinji Ueno
    Ophthalmology, Nagoya Univ School of Med, Nagoya, AICHI, Japan
  • Satoshi Okado
    Ophthalmology, Nagoya Univ School of Med, Nagoya, AICHI, Japan
  • Taro Kominami
    Ophthalmology, Nagoya Univ School of Med, Nagoya, AICHI, Japan
  • Daiki Inooka
    Ophthalmology, Nagoya Univ School of Med, Nagoya, AICHI, Japan
  • Ayami Nakanishi
    Ophthalmology, Nagoya Univ School of Med, Nagoya, AICHI, Japan
  • Akira Sayo
    Ophthalmology, Nagoya Univ School of Med, Nagoya, AICHI, Japan
  • Mineo Kondo
    Mie University Graduate School of Medicine, Tsu, Japan
  • Hiroko Terasaki
    Ophthalmology, Nagoya Univ School of Med, Nagoya, AICHI, Japan
  • Footnotes
    Commercial Relationships   Shinji Ueno, None; Satoshi Okado, None; Taro Kominami, None; Daiki Inooka, None; Ayami Nakanishi, None; Akira Sayo, None; Mineo Kondo, None; Hiroko Terasaki, None
  • Footnotes
    Support  JSPS KAKENHI Grant Number (JP16K11320 SU)
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 5529. doi:
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    • Get Citation

      Shinji Ueno, Satoshi Okado, Taro Kominami, Daiki Inooka, Ayami Nakanishi, Akira Sayo, Mineo Kondo, Hiroko Terasaki;
      Temporal properties of flicker ERGs in a transgenic rabbit model of retinitis pigmentosa. Invest. Ophthalmol. Vis. Sci. 2018;59(9):5529.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To determine the effects of a remodeled inner retina on the flicker ERGs in a rabbit eye at an advanced stage of retinal degeneration.

Methods :
Six wild-type (WT) and four rhodopsin P347L transgenic (Tg) rabbits were studied at 18-months of age. Flicker ERGs were elicited by sinusoidal stimuli at frequencies of 3.906 Hz to 50.781 Hz in 13 steps. To block ON and OFF retinal pathways, 2-amino-4-phosphonobutyric acid (APB), and 6-cyano-7-nitroquinoxaline-2, 3(1H, 4H)-dione (CNQX) were injected intravitreally. The amplitudes and phases of the fundamental components of the pre- and post-drug ERGs were analyzed. The postsynaptic APB (ON-) and CNQX (OFF-) sensitive components were determined by examining the phase and amplitude vectors.

Results : The temporal properties of the Tg rabbits were different from those of the WT rabbits and had unique features; at frequency of 3.906 Hz, the amplitude was depressed but it increased by more than 3.5-fold at 15.625 Hz. The reduction of the amplitude at 3.906 Hz in Tg rabbits was caused by a cancelation of the ON and OFF components by a phase difference of 180°. On the other hand, an increase in the amplitude at 15.625 Hz in Tg rabbits was caused by the summation of the ON and OFF components which had an approximate 120° phase difference.

Conclusions : The temporal properties of the flicker ERGs of Tg rabbits were markedly affected by the remodeling of the retinal neurons. Evaluations of the flicker ERGs in RP eyes needs to be done with careful considerations of the current findings.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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