July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Aβ-potentiated and Aβ-independent age-related changes in the lens of Alzheimer’s Disease and wild-type mice
Author Affiliations & Notes
  • Juliet A Moncaster
    Radiology, Boston University, Boston, Massachusetts, United States
  • Mark Wojnarowicz
    Pathology and Laboratory Medicine, Boston University, Boston, Massachusetts, United States
  • Olga Minaeva
    Radiology, Boston University, Boston, Massachusetts, United States
  • Rebecca Zeng
    Radiology, Boston University, Boston, Massachusetts, United States
  • Lee E Goldstein
    Radiology, Boston University, Boston, Massachusetts, United States
  • Footnotes
    Commercial Relationships   Juliet Moncaster, None; Mark Wojnarowicz, None; Olga Minaeva, None; Rebecca Zeng, None; Lee Goldstein, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 5640. doi:https://doi.org/
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    • Get Citation

      Juliet A Moncaster, Mark Wojnarowicz, Olga Minaeva, Rebecca Zeng, Lee E Goldstein; Aβ-potentiated and Aβ-independent age-related changes in the lens of Alzheimer’s Disease and wild-type mice. Invest. Ophthalmol. Vis. Sci. 2018;59(9):5640. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Crystallins comprise ~90% of lens protein in mature lens fiber cells and undergo various post-translational modifications during aging that disrupt the normal functioning of the proteins, facilitating aggregation, insolubilization and light scattering. Crystallins have been shown to interact with Aβ in particular in Alzheimer’s disease. Here, we expand on previous work by us and others and investigated the aging effect of crystallins in an Aβ-independent and Aβ-potentiated environment and the effect on light scattering in lenses from aged wild-type and Alzheimer’s disease transgenic mice (APPswe).

Methods : Mice were bred, maintained and genotyped at Boston University. Mice were sacrificed at ages 13-27 months of age, perfused with phosphate buffer saline, lenses were isolated and then imaged under two different sources of light using a D70 digital Nikon camera and a custom-adapted Zeiss stereophotomicroscope.

Results : Wild-type aged mice demonstrated light scattering in two distinct outer cortical and inner cortical regions of the lens at 27 months compared to younger mice. Furthermore, APPswe aged mice showed increased scattering in the cortical layer at 27 mths compared to wild-type aged mice.

Conclusions : Two distinct cortical light scattering regions of the lens are affected during aging involving Aβ-potentiated mechanisms in APPswe mice and Aβ-independent mechanisms in wild-type mice.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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