July 2018
Volume 59, Issue 9
Free
ARVO Annual Meeting Abstract  |   July 2018
Co-delivery of Dexamethasone-Melatonin-CoQ10 from a microparticulate drug delivery system. Potential usefulness in neuroprotective therapy.
Author Affiliations & Notes
  • Alicia Arranz-Romera
    Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy(UCM920415), Complutense University of Madrid, Madrid, Spain
    Red Temática de Investigación Cooperativa Sanitaria en Enfermedades Oculares Oftared e Instituto de Investigación Sanitaria del Hospital Clínico San Carlos, Madrid, Spain
  • Ehtesham Shamsher
    Department of Visual Neuroscience, Glaucoma and Retinal Neurodegeneration Rearch,UCL Institute of Ophthalmology, London, United Kingdom
  • Sergio Esteban-Pérez
    Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy(UCM920415), Complutense University of Madrid, Madrid, Spain
    Red Temática de Investigación Cooperativa Sanitaria en Enfermedades Oculares Oftared e Instituto de Investigación Sanitaria del Hospital Clínico San Carlos, Madrid, Spain
  • Ben Davis
    Department of Visual Neuroscience, Glaucoma and Retinal Neurodegeneration Rearch,UCL Institute of Ophthalmology, London, United Kingdom
  • David Garcia-Herranz
    Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy(UCM920415), Complutense University of Madrid, Madrid, Spain
  • Li Guo
    Department of Visual Neuroscience, Glaucoma and Retinal Neurodegeneration Rearch,UCL Institute of Ophthalmology, London, United Kingdom
  • Irene T. Molina-Martínez
    Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy(UCM920415), Complutense University of Madrid, Madrid, Spain
    Red Temática de Investigación Cooperativa Sanitaria en Enfermedades Oculares Oftared e Instituto de Investigación Sanitaria del Hospital Clínico San Carlos, Madrid, Spain
  • Irene Bravo-Osuna
    Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy(UCM920415), Complutense University of Madrid, Madrid, Spain
    Red Temática de Investigación Cooperativa Sanitaria en Enfermedades Oculares Oftared e Instituto de Investigación Sanitaria del Hospital Clínico San Carlos, Madrid, Spain
  • M Francesca Cordeiro
    Department of Visual Neuroscience, Glaucoma and Retinal Neurodegeneration Rearch,UCL Institute of Ophthalmology, London, United Kingdom
    Western Eye Hospital, Imperial College London, London, United Kingdom
  • Rocio Herrero-Vanrell
    Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy(UCM920415), Complutense University of Madrid, Madrid, Spain
    Red Temática de Investigación Cooperativa Sanitaria en Enfermedades Oculares Oftared e Instituto de Investigación Sanitaria del Hospital Clínico San Carlos, Madrid, Spain
  • Footnotes
    Commercial Relationships   Alicia Arranz-Romera, None; Ehtesham Shamsher, None; Sergio Esteban-Pérez, None; Ben Davis, None; David Garcia-Herranz, None; Li Guo, None; Irene T. Molina-Martínez, None; Irene Bravo-Osuna, None; M Francesca Cordeiro, None; Rocio Herrero-Vanrell, None
  • Footnotes
    Support  Spanish Ministry of Economy and Competitiveness (MAT 2013-42127-R): predoctoral fellowship (BES-2014-070041), ISCIII-FEDER “Una manera de hacer Europa” RETICS: RD16/0008, and Research Group (UCM 920415).
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 5693. doi:
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    • Get Citation

      Alicia Arranz-Romera, Ehtesham Shamsher, Sergio Esteban-Pérez, Ben Davis, David Garcia-Herranz, Li Guo, Irene T. Molina-Martínez, Irene Bravo-Osuna, M Francesca Cordeiro, Rocio Herrero-Vanrell; Co-delivery of Dexamethasone-Melatonin-CoQ10 from a microparticulate drug delivery system. Potential usefulness in neuroprotective therapy.. Invest. Ophthalmol. Vis. Sci. 2018;59(9):5693.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Design and characterization of a novel microparticulate Drug Delivery System (DDS) loaded with three active substances (Dexamethasone, Melatonin and Coenzyme Q10) with potential use in neuroprotective therapy.

Methods : Poly(lactic-co-glycolic) (PLGA) acid microspheres (MPs) loaded with Dexamethasone (DX), Melatonin (MEL) and Coenzyme Q10 (CoQ10) were prepared according to the extraction-evaporation technique from an Oil/Water emulsion. Microspheres were characterized in terms of morphology, mean particle size, encapsulation efficiencies and in vitro release studies. The amount of drugs encapsulated and released from the microspheres were quantified by HPLC-MS. A retinal cell line (RC28) was used as in vitro model for toxicity and bioactivity studies.

Results : PLGA microspheres ranged from 20-30 µm in size and were spherical in shape with rough surfaces. The microencapsulation efficiencies resulted 78.20±0.42 % (115.85±0.62 µg DX/mg MPs), 61.83±2.51% (45.80±1.86µg MEL/mg MPs) and 96.42±4.12% (35.71±1.53µg CoQ10/mg MPs). The active agents were released in vitro in a controlled fashion up to 30 days. The DX/MEL/CoQ10-MPs significantly promoted survival (p<0.05) of retinal cells from glutamate excitoxicity (IC50: 10.00±0.94) compared with non-loaded-MPs (IC50: 6.35±1.91) and control (IC50: 6.89±0.82).

Conclusions : The novel multiloaded microparticulate Drug Delivery System showed potential usefulness in neuroprotective therapy.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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