July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
The effects of amniotic membrane transplantation on moxifloxacin delivery into the cornea evaluated by in-vivo two-photon microscopy
Author Affiliations & Notes
  • jin longyu
    School of medicine, Dong-A University, Busan, Korea (the Republic of)
  • Woo Park
    School of medicine, Dong-A University, Busan, Korea (the Republic of)
  • Woo Jin Jeong
    School of medicine, Dong-A University, Busan, Korea (the Republic of)
  • Ki Hean Kim
    Pohang Unuiversity of science and technology, Pohang, Korea (the Republic of)
  • Uk Jegal
    Pohang Unuiversity of science and technology, Pohang, Korea (the Republic of)
  • Jun Ho Lee
    Pohang Unuiversity of science and technology, Pohang, Korea (the Republic of)
  • Footnotes
    Commercial Relationships   jin longyu, None; Woo Park, None; Woo Jin Jeong, None; Ki Hean Kim, None; Uk Jegal, None; Jun Ho Lee, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 5700. doi:https://doi.org/
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      jin longyu, Woo Park, Woo Jin Jeong, Ki Hean Kim, Uk Jegal, Jun Ho Lee; The effects of amniotic membrane transplantation on moxifloxacin delivery into the cornea evaluated by in-vivo two-photon microscopy. Invest. Ophthalmol. Vis. Sci. 2018;59(9):5700. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To investigate how the amniotic membrane transplantation affects moxifloxacin penetration into mouse cornea by using in-vivo two-photon microscopy.

Methods : Measurement was conducted in mouse corneas in vivo, and Balb/c female mice aged 5-6 weeks were used. Ten mice were divided into two groups, with amniotic membrane transplantation (AMT group) and without amniotic membrane (control group). Mouse eyes in AMT group had amniotic membrane (AM) sutured on the cornea. Moxifloxacin eye drop (Vigamox, Alcon, TX, USA) was topically administered on the AM of AMT group and the cornea of control group, and the eyes were wash out with PBS after 5 minutes. Spatial distributions of moxifloxacin was measured by two-photon microscopy based on moxifloxacin intrinsic fluorescence every 10 minutes until 1 hour. Fluorescence excitation wavelength was 790 nm for moxifloxacin.

Results : In AMT group, moxifloxacin was detected in the cornea in 10 minutes after instillation. AMT group showed more than 20% of moxifloxacin in the mouse cornea in 10 minutes after instillation compared to the moxifloxacin intensity in control group. AM seemed to transport topically instilled moxifloxacin to the cornea without much resistance. At later time points, moxifloxacin intensities in the corneas decreased exponentially with time via diffusion. The slope of moxifloxacin intensity decay with time was similar in both AMT and control group at early time points until 20 minutes after instillation, but the decaying slope in AMT group became less stiff at later time points compared to the one in control group. AMT group seemed to keep moxifloxacin in the cornea for the longer duration compared to control group, and the transplanted AM might work as a reservoir.

Conclusions : Moxifloxacin eye drop penetrated well into the cornea through the transplanted amniotic membrane, and the transplanted amniotic membrane worked as a reservoir and released moxifloxacin for longer duration.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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